摘要
目的观察痰热清注射液、喜炎平注射液与头孢他啶注射液联合作用时对铜绿假单胞菌的体外抗菌效果。方法采用96孔板微量肉汤稀释法单独测定痰热清注射液、喜炎平注射液和头孢他啶注射液的体外最低抑菌浓度(MIC);采用棋盘格联合药敏试验法测定痰热清注射液,喜炎平注射液联合头孢他啶注射液时对铜绿假单胞菌的抑菌浓度;对联合抑菌浓度与单独抑菌浓度进行比较分析。结果头孢他啶注射液在0.125μg/mL;喜炎平注射液在0.625μg/mL时为单独药敏最低抑菌浓度;痰热清注射液在1∶16稀释倍数时为单独药敏最低稀释度。头孢他啶稀释倍数1∶256;痰热清注射液稀释倍数1∶64为联合药敏最低抑菌稀释度。头孢他啶稀释倍数1∶256;喜炎平注射液稀释倍数1∶16为联合药敏最低抑菌稀释度。注射用头孢他啶联合痰热清注射液分级抑菌浓度指数(FIC)为0.375,注射用头孢他啶联合喜炎平注射液FIC指数为0.625。结论注射用头孢他啶联合痰热清注射液为协同作用,注射用头孢他啶联合喜炎平注射液为相加作用。痰热清注射液,喜炎平注射液与头孢他啶注射液联合均起联合抑菌作用,其中痰热清注射液作用最强。
Objective: To compare the anti-bacterial effect in vitro of Tanreqing Injection and Xiyanping Injection combined with Ceftazidime Injection on Pseudomonas aeruginosa. Methods: Minimum inhibitory concentration(MIC) in vitro of Tanreqing Injection,Xiyanping Injection and Ceftazidime Injection was detected with 96 hole plate micro broth dilution method. MIC of Tanreqing Injection,Xiyanping Injection and Ceftazidime Injection on Pseudomonas aeruginosa was detected with the method of checkerboard combined with drug sensitivity test.Comparative analysis was carried out between the united inhibitory concentration and separate inhibitory concen-tration. Results: Ceftazidime Injection at 0.125 μg/mL and Xiyanping Injection at 0.625 μg/mL was the minimum inhibitory concentration of individual drug sensitivity,as well as Tanreqing Injection diluted at 1∶16. Ceftazidime dilution multiple 1:256 and Tanreqing Injection dilution multiple 1∶64 was the minimum inhibitory concentration of combined drug sensitivity. Ceftazidime dilution multiple 1 ∶256 and Xiyanping Injection dilution multiple 1 ∶16 was the minimum inhibitory concentration of combined drug sensitivity. FIC of Ceftazidime Injection combined with Tanreqing Injection was 0.375,and that of Ceftazidime Injection combined with Xiyanping Injection was0.625. Conclusion: There is a synergistic action in Ceftazidime Injection combined with Tanreqing Injection,and an additive effect in Ceftazidime Injection combined with Xiyanping Injection. Tanreqing Injection and Xiyanping Injection combined with Ceftazidime Injection can play the combined inhibitory effect,and the strongest effect comes from Tanreqing Injection.
出处
《中国中医急症》
2018年第2期296-299,共4页
Journal of Emergency in Traditional Chinese Medicine
基金
上海中西医结合医院院内科研基金项目(中西医16-02-02)
