摘要
目的:观察芝麻素对肥大细胞活化和炎症介质释放的影响并探讨其可能的作用机制。方法:体外培养HMC-1细胞,用10μg/ml的anti-DNP Ig E处理细胞6 h后,再用100 ng/ml的DNP-HAS孵育10 min诱导HMC-1细胞活化,在DNPHAS处理前给予最终浓度25、50和100μg/L芝麻素预处理30 min后光镜下观察芝麻素对肥大细胞脱颗粒的影响,ELISA法检测芝麻素对肥大细胞组胺、TNF-α、IL-6、IL-1β、IL-8释放的影响,Western blot方法观察芝麻素对FcεRI受体下游信号Lyn和Syk,PKCα激活,IκBα磷酸化和NF-κB活化的影响。结果:DNP-HAS能明显诱导HMC-1细胞脱颗粒,促进组胺和TNF-α、IL-6、IL-1β和IL-8等细胞因子的释放,激活FcεRI受体下游信号分子Lyn、Syk和PKCα,磷酸化IκBα,促进NF-κB活化(P<0.05)。芝麻素高(100μg/L)、中(50μg/L)浓度能明显抑制HMC-1细胞脱颗粒和炎症介质的释放,阻断FcεRI受体下游信号激活,抑制NF-κB活化(P<0.05)。结论:芝麻素通过PKCα/NF-κB途径抑制肥大细胞活化和炎症介质释放。
Objective： To investigate the effect of sesamin on mast cell activation and its inflammatory mediator release,as well as its possible mechanisms of action. Methods： HCM-1 cells were activation by stimulation with 10 μg/ml anti-DNP Ig E for 6 h and challenge with 100 ng/ml DNP-HAS for 10 min. Sesamin was administration at the concentration of 25,50 and 100 μg/L prior to DNPHAS challenge,subsequently the effect of sesamin on mast cell degranulation was investigated by light microscope,and histamine release and expression of cytokines such as TNF-α IL-6,IL-1β,IL-8 of mast cells after sesamin treatment were investigated by ELISA. Western blot was used to determine the effect of sesamin on FcεRI downstream signaling including Lyn,Syk and PKCα activation,and IκBαphosphorylation and NF-κB activation. Results： DNP-HAS significantly increased mast cell degranulation,histamine release and those cytokines expression,enhanced Lyn,Syk,PKCα,IκBα phosphorylation and NF-κB activation（ P〈0. 05）. Sesamin（ 50,100 μg/L） significantly decreased mast cell degranulation,histamine release and cytokines expression（ TNF-α,IL-4,IL-1β,and IL-8）,reduced activity of Lyn,Syk,kinases and PKCα and IκBα phosphorylation,and inhibited NF-κB activation（ P〈0. 05）. Conclusion： Sesamin suppresses mast cell activation and inflammatory mediators release through inhibition of PKCα/NF-κB signaling pathway.
作者
赵洪伟
崔银锋
姜京植
车楠
叶晶
王重阳
李良昌
ZHAO Hong-Wei;CUI Yin-Feng;JIANG Jing-Zhi;CHE Nan;YE Jing;WANG Chong-Yang;LI Liang-Chang(Yanbian University Medical College,Yanji 133002,China)
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2018年第2期167-171,共5页
Chinese Journal of Immunology
基金
国家自然科学基金项目(81560004)
吉林省科技厅项目(20160101210JC)资助
