摘要
为提高果胶酯酶的产量,以果胶酯酶活力与生物量为指标,对塔宾曲霉CICC 2651产果胶酯酶的发酵培养基和培养条件进行了研究,通过单因素实验得到最优培养基和培养条件为:硫酸铵0.5%,果胶3%,Na_2SO_40.04%,Mg SO_4·7H_2O 0.04%,K_2HPO_40.2%,培养温度30℃,初始p H为4.5,接种量5%,装液量40 m L。在优化工艺条件下,果胶酯酶活力达到1.53±0.09 U/m L,比初始条件提高了77.9%。通过发酵培养基和培养条件优化,塔宾曲霉CICC 2651发酵产果胶酯酶的能力大幅度提高。
In order to increase the yield of pectin esterase,the medium compositon and culture conditons were studied for fermentation of Aspergillus tubingensis CICC 2651.Resulted in biomass and pectin esterase activity,the optimal medium compostion was composed of high-ester pectin 3%,ammonium sulfate 0.5%,Na2SO40.04%,Mg SO4·7H2O 0.04%,K2HPO40.2%,culture temperature 30℃,initial p H4.5,inculation volume 5%,liquid volume 40 m L. Compared to the basic technical contidtions,the enzyme activity at the optimum technique reached 1.53 ± 0.09 U/m L,and increased by 1.79-fold. Under the optimum fermentation process,the production capability of pectin esterase by Aspergillus tubingensis CICC 2651 was significantly improved.
出处
《食品工业科技》
CAS
CSCD
北大核心
2018年第3期82-86,91,共6页
Science and Technology of Food Industry
基金
福建省高校产学合作项目(2016N5008)
福建省科技重大专项项目(2015NZ0001-1)
福建省科技重点项目(2014Y0081)
国家海洋公益行业科研专项(201505033)
福建省海洋高新产业发展专项(闽海洋高新[2016]08号)资助
关键词
果胶酯酶
发酵培养基
培养条件
发酵优化
酶活
pectin esterase
medium composition
culture conditions
fermentation optimization
enzyme activity
