摘要
本研究根据Gen Bank上发表的鸭瘟病毒(DPV)基因序列设计了1对引物,分别对DPV AV 1221株和DPV鸡胚化弱毒疫苗株的TK基因进行扩增,扩增的目的片段克隆至载体p MD19-T,经PCR和双酶切(Bam HⅠ+HindⅢ)鉴定,将阳性质粒进行测序和生物信息学分析。结果成功克隆出了长度均为1 077 bp的DPV TK基因,生物信息学分析结果表明两个片段的同源性达到100%。
According to the sequence of TK gene in GenBank, a pair of primers was designed to amplify this gene in virulent and vaccine duck plague virus strains. The purpose amplified fragment was cloned into pMD 19-T vector. The recombinant expression vector was selected and identified by PCR and restriction enzyme digestion （BarnH I ＋Hind llI ）. The positive recombinant plasmid was then sequenced and the biological information was also analyzed. The results showed this research successfully cloned the DPV TK gene, and both of the fragments were 1 077 bp, the result of bioinformaties analysis revealed the ho- mology of two fragments up to 100%.
出处
《四川畜牧兽医》
2018年第2期31-33,共3页
Sichuan Animal & Veterinary Sciences
基金
国家农业科技成果转化资金项目--国家科技部农业项目"规模化养鸭场标准化健康养殖技术示范与推广"(2013GB2F000422)
