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人体尿液和血浆中碘元素形态分析方法学的研究 被引量:6

A study of iodine species in human urine and plasma
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摘要 目的研究碘元素在人体尿液和血浆中的形态。方法分别建立离子色谱-电感耦合等离子体质谱联用(IC-ICPMS)、尺寸排阻色谱-电感耦合等离子体质谱联用(SEC-ICP-MS)方法,分析人体尿液和血浆中碘元素形态及含量。结果 ICICP-MS法可对IO3-和I-定量测定,检出限均为0.3ng/mL(均以碘计),0.5~150ng/mL范围内线性相关系数r>0.999。实际人体尿液和血浆中均未发现IO3-,尿液中只有I-,I-相对标准偏差(RSD)为4.7%,加标回收试验回收率为92%~105%。SEC-ICP-MS法证明血浆中碘主要吸附于血浆中蛋白或大分子物质上,同时存在部分游离I-,利用SEC-ICP-MS定量测定血浆中游离I-,方法检出限为0.6ng/mL,2.0~50.0ng/mL范围内线性相关系数r>0.999,血浆中I-RSD为6.4%,血浆加标回收试验回收率为82%~94%。结论建立的离子色谱-电感耦合等离子体质谱联用方法,可测定人体尿液中碘元素形态及含量,建立的尺寸排阻色谱-电感耦合等离子体质谱联用方法,可测定人体血浆中碘元素形态及I-的含量。 Objective To study iodine species in human urine and plasma.Methods A method based on ion chromatography-inductively coupled plasma mass spectrometry(IC-ICP-MS)had been established to study iodine species and quantities in human urine;a method based on size exclusion chromatography-inductively coupled plasma mass spectrometry(SEC-ICP-MS)was developed to study iodine species and quantities in human plasma.Results IC-ICP-MS determined the IO3-and I-quantitatively,the limits of detection(LOD)were 0.3 ng/mL for both IO3-and I-(calculated by I).The linear coefficients of IO3-and I-and were all〉0.999 in the concentration range of 0.5-150 ng/mL.IO3-was not detected neither in plasma nor urine,while I-was only detected in urine.The relative standard deviation(RSD)of I-in urine was 4.7% while the spiked recovery rate was in the range of 92%to 105%.SEC-ICP-MS analysis demonstrated that iodine in plasma was mostly bounded to protein and large molecules with certain amount of I-.The I-in plasma was quantitatively determined by SEC-ICP-MS with the LOD of 0.6 ng/mL.The linear coefficient of I-standard curve was〉0.999 in the concentration range of 2.0-50.0 ng/mL.The spiked recovery rate was in the range of 82%to 94%.Conclusion The established IC-ICP-MS assay can analyze iodine species and quantities in human urine,while the established SEC-ICP-MS can analyze the iodine species and I-quantity in human plasma.
作者 王媛 刘德晔
出处 《江苏预防医学》 CAS 2018年第1期1-3,60,共4页 Jiangsu Journal of Preventive Medicine
基金 "十三五"江苏省医学青年人才项目(QNRC2016540) 江苏省预防医学科研课题(Y20130053)
关键词 离子色谱 电感耦合等离子体质谱 尺寸排阻色谱 碘元素 形态分析 尿液 血浆 Ion chromatography Inductively coupled plasma mass spectrometry Size exclusion spectrometry Iodine Speciation analysis Urine Plasma
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