摘要
针对超分辨成像方法景深不够的局限性,发展了具有超大景深的三维超分辨成像系统,通过点扩展函数的改造,形成携带有三维信息的双螺旋点形式,同时结合变形光栅能够在12μm轴向范围内实现荧光分子的实时位置信息获取,为了精确地获得荧光分子的定位,利用模式识别方法实现图像重构,通过系统标定获取匹配图像,利用相关性计算以及拟合算法获取分子的三维位置信息。实验验证,该方法可以高效实现图像信息获取,为DH-PSF与DG结合方法实现三维成像奠定了基础。
As the conventional super-resolution method has limited depth of field,we have developed a 3D super-resolution imaging system with extended depth of field. The system can recognize the real-time position of the fluorescent probes within the axial range of 12 μm,which uses the transformation of PSF to form double helix points,and simultaneously combines the distorted grating. In order to obtain an accurate localization of fluorescent probes,we use template matching method to realize the image reconstruction. The matching image is obtained through system calibration,and the 3D position of probes is obtained by the correlation and fitting algorithm. Experimental results shows that our system has high efficiency to obtain image information,and lays the foundation for the realization of 3D imaging combined with DH-PSF and DG.
出处
《电子显微学报》
CAS
CSCD
北大核心
2018年第1期64-70,共7页
Journal of Chinese Electron Microscopy Society
基金
国家重点基础研究发展计划(973计划)(No.2012CB825802)
国家自然科学基金资助项目(Nos.61605127,11004136,61235012,61178080)
广东省自然科学基金资助项目(No.2014A030312008)
深圳市科技计划资助项目(Nos.GJHS20120621155433884,JCYJ20120613173049560,JCYJ20150324141711698)
关键词
三维成像
双螺旋点扩展函数
变形光栅
模式识别
动态追踪
three dimensional imaging
double helix point spread function
distorted grating
template matching
dynamic tracking
