摘要
采用外观解剖观察和组织学方法,对克氏原螯虾雌性的生殖系统发育及组织结构进行了研究。结果表明:克氏原螯虾的雌性生殖系统由卵巢、输卵管组成,卵巢呈"Y"型;卵巢发育早期分布有卵室,分化生殖卵原细胞。卵子发生经历卵原细胞、卵黄合成前卵母细胞、卵黄合成期卵母细胞及成熟期卵母细胞4个时相;克氏原螯虾卵巢发育期可分为Ⅰ期、Ⅱ期、Ⅲ期(又分为早、中、晚期)、Ⅳ期、Ⅴ期5个时期。卵质内含有滑面内质网、致密小颗粒物质、线粒体,参与卵黄颗粒的合成。将卵巢呈黑色、卵黄充满卵母细胞、细胞核消失、卵母细胞与滤泡细胞分离等特征作为克氏原螯虾成熟的标志。克氏原螯虾个体性腺发育不同步,每年产卵1次,群体每年存在两个产卵高峰期,一个在9~10月份,另一个在4~5月份。
The development of female reproductive system and histological structure of Procambarus clarkii were studied by using appearance anatomic observation and histological method. The results showed that the female reproductive system of P. clarkii consisted of ovaries and oviduct,and ovaries were Y-shaped. Egg chambers were distributed in ovaries at their earlier development stage,and the oocytes were reproduced in egg chamber. The oogenesis experienced the following four phases： oogoniums,pre-yolk-synthesis oocytes,yolk-synthesis oocytes,and mature oocytes. The ovary development of P. clarkii could be divided into five stages： stage Ⅰ,stage Ⅱ,stage Ⅲ（including early stage,middle stage,and later stage）,stage Ⅳ,and stage Ⅴ. The ooplasms contained smooth endoplasmic reticulum,dense small-particulate matter and mitochondria,which were involved in the synthesis of yolk granules. The characteristics of black ovaries,filling oocytes with egg yolks,nucleus disappearing,and oocytefollicular cell separation were used as the mature signs of this crayfish. The gonadal development of P. clarkii individuals was not synchronized,they spawned once a year,and the population had two spawning peak periods each year： from September to October,and from April to May.
出处
《江西农业学报》
CAS
2018年第2期68-75,共8页
Acta Agriculturae Jiangxi
基金
安徽省农业科学院院长青年创新基金项目(15B0509)
安徽省水产产业技术体系(皖农科[2016]84号)
关键词
克氏原螯虾
雌性生殖系统
发育
组织结构
Procambarus clarkii
Female reproductive system
Development
Organizational structure
