摘要
对分离自国内虎源的犬瘟热病毒进行细胞培养及PCR鉴定,并对H蛋白进行序列及遗传演化分析。结果显示:本分离株经传代培养后可以引起Vero细胞脱落和死亡,且扩增出580 bp的特异性目的片段。H蛋白作为犬瘟热病毒表面膜蛋白,在病毒感染过程中担负着重要的作用,H蛋白识别并吸附于细胞表面受体,决定病毒细胞嗜性,进而决定病毒的宿主特异性。对本研究中H蛋白进行分析发现:全长共1 824个碱基,共计编码608个氨基酸,H蛋白具有9个N-糖基化位点,具有549Y的特点。在Genbank中进行Blast分析表明,分离株的H序列与登录号为AF178038的小熊猫源、登录号为KM386683的东北虎源犬瘟热分离株的核苷酸序列相似性最高,均为99%。下载犬瘟热相关序列进行遗传演化、氨基酸序列比对分析,发现本分离株的H基因与标准强毒株A75/17的氨基酸同源性为96.2%,与疫苗Onderstepoort株的氨基酸同源性为89.8%。遗传演化分析显示本分离株基因型属于Asia-1型,与我国流行的野毒株相似。
We cultured the canine distemper virus isolated from siberian tiger in China and identified by PCR, and analyzed the sequence and genetic evolution of H protein. The results showed that the subcultured isolate could cause Vero cells to shed and die, and the specific fragment of 580 bp sequence could be amplified. H protein, as the surface membrane protein of canine distemper vi- rus, plays important role in the process of virus infection. H protein recognizes and is bound to the receptor on the cell surface. It determines the preferendum of virus to cell, therefore determines the host specificity of the virus. The analysis of H protein gene showed that the total length was 1 824 bases, encoding 608 amino acids, and H protein had 9 N-glycosylation sites, which had the characteristics of 549Y. Blast analysis in Genbank＇M showed that the nucleotide sequence of the i- solate H protein was most similar between isolates from red panda（accession number AF178038） and isolates from siberian tiger （accession number KM386683）, The similarities of those se- quences were both 99%. Analysis of genetic evolution and amino acid sequence with the related sequences of canine distemper virus showed that the amino acid homology of the H gene from this isolate was 96. 2% with that of the standard strain A75/17, and was 89.8% with the Strain Onder- stepoort. Genetic evolution analysis showed that the isolate belonged to Type Asia-1, similar to those of wild strains in China.
出处
《野生动物学报》
北大核心
2018年第1期35-40,共6页
CHINESE JOURNAL OF WILDLIFE
关键词
犬瘟热病毒
血凝素基因
基因型
进化分析
Canine distemper virus
Haemagglutinin （ H ） gene
Genotype
Phylogenetic analysis
