摘要
目的对塞曼原子吸收汞分析仪测定尿汞的方法进行评价,为实验检测及临床诊断中选用简便、快捷的测汞方法提供参考。方法采用塞曼原子吸收光谱法(ZAAS)和氢化物发生-原子吸收光谱法(HG-AAS)进行尿汞含量测定,对两种方法的检出限、准确度、精密度及一致性进行比较。结果 ZAAS在0~1000 ng/m L范围内呈良好的线性关系(R2=1.0000),HG-AAS在0~20 ng/m L范围内呈良好的线性关系(R2=0.9990)。ZAAS和HG-AAS检出限分别为0.156 ng/m L、1.593 ng/m L,ZAAS灵敏度较高。ZAAS加标回收率在97.5%~103.2%之间,HG-AAS加标回收率在95.6%~104.5%之间;重复测量10 ng/m L、100 ng/m L汞标准溶液10次,ZAAS的相对标准偏差(RSD)分别为0.30%和0.36%,HG-AAS的RSD分别为2.82%和1.11%,两种方法准确度和精密度均满足GBZ/T 210.5-2008标准的要求,ZAAS精密度较高。用两种方法对30份尿液样本进行检测,经配对t检验,差异无显著性(P>0.05),两种方法检测结果有较高一致性(R2=0.9961)。结论 ZAAS能够快捷、准确测量尿汞含量,检出限较低、精密度较高。
Objective To evaluate the method for detection of urinary mercury using a Zeeman atomic absorption mercury analyzer and to provide a reference for selecting a convenient method for mercury detection in experiments and clinical diagnoses. Methods Urinary mercury was detected by Zeeman atomic absorption spectroscopy（ ZAAS） and hydride generation atomic absorption spectrometry（ HG-AAS）, and the detection limit, accuracy, precision and consistency of the two methods were compared. Results The Data collected by ZAAS and HG-AAS showed a good linear relationship in the range of 0-1000 ng/m L（ ZASS,R2= 1. 0000） and 0-20 ng/m L（ HG-AAS,R2= 0. 9990）. The detection limits of ZAAS was 0. 156 ng/m L and that of HG-AAS was 1. 593 ng/m L,indicating that ZAAS is more sensitive. The recovery rate of standard addition of ZAAS was between 97. 5% and 103. 2%,and that of HG-AAS was between 95. 6% and 104. 5%. After measurement of 10 ng/m L and 100 ng/m L mercury standard solutions repeated for 10 times,the relative standard deviation（ RSD） of ZAAS was 0. 30% and 0. 36% respectively,and the RSD of HG-AAS was2. 82% and 1. 11%,respectively. The accuracy and precision of both the two method met the standards of GBZ/T 210. 5-2008,and the precision of ZAAS was better. A total of 30 urine samples were measured by these two methods. The results were compared with paired-samples t-test and showed a non-significant difference（ P〈0. 05）, indicating a high consistency of these two method（ R2= 0. 9961）. Conclusions ZAAS is a convenient and accurate method for the detection of urinary mercury,with a relatively low detection limit and better precision.
出处
《中国比较医学杂志》
CAS
北大核心
2018年第1期86-90,共5页
Chinese Journal of Comparative Medicine
基金
国家自然科学基金项目(编号:81503209)
辽宁省教育厅一般项目(编号:L2015320)
关键词
塞曼原子吸收光谱法
氢化物发生-原子吸收光谱法
尿汞
方法学评价
Zeeman atomic absorption spectroscopy, ZAAS
hydride generation atomic absorption spectrometry,HG-AAS
urinary mercury
methodological evaluation
