摘要
目的探讨建立小鼠梗阻性结肠癌模型的方法,并验证其稳定性。方法将50只BALb/c小鼠随机分成25只梗阻性结肠癌模型组(研究组)和25只PBS替代癌细胞模型组(对照组),制模后各组小鼠再分成5个亚组,分别在制模3天、7天、10天、14天、21天处死,并采用小鼠一般状态标注法、拟临床癌性肠梗阻监测法及病理学方法比较两组差异。结果研究组制模成瘤率100%(25/25)。在小鼠模型一般状态的数据分析中,发现研究组与对照组相比在制模后10天起平均进食量、体重及粪便量明显减少,其差异有统计学意义(P<0.05)。在拟临床癌性肠梗阻监测数据分析中,发现研究组在制模后7天起平均腹围、肠道内压力及梗阻上段肠管周径明显高于对照组,其差异有统计学意义(P<0.05)。在病理学检测数据分析中,发现研究组在制模后7 d起成瘤,制模后14天起肿瘤环腔生长,并逐渐出现梗阻性结肠癌样改变。结论小鼠梗阻性结肠癌模型的建立方法简便、肠管损伤程度小、可行、安全、死亡率低,且制模稳定性高;反映了临床梗阻性结肠癌的病理生理演变过程,为较理想的动物模型。
Objective To establish of obstructive colon cancer model in BALb/c mice with method and verify its stability.Methods The 50 BALb/c mice were randomized into two groups,25 obstructive colon cancer model group（study group） and 25 PBS replaces cancer cells model group（control group）.After the model,the mice were divided into five sub-groups,and executed in postoperative 3,7,10,14 and 21 day respectively.BALb/c mice of general conditions,simulative cancerous intestinal obstruction of surveilance method,and histopathological examination were evaluated in two groups.Results The rates of tumor formation was 100.0%（25/25） in the study group.BALb/c mice model of general conditions of data analysis,study group had a significantly losses mean food intake,weight,and excrement in the postoperative 10 days（P〈0.05）.The simulative cancerous intestinal obstruction of surveilance method of data analysis,study group showed significantly mean increase obdominal circumference,intestinal pressure,and circumference of the intestinal which located at upper the obstruction site in the postoperative 7 days（P〈0.05）.The histopathological examination of data analysis,tumor formation time was postoperative 7 days,and colon circumferential growth and obstructive colon cancer of times were postoperative 14 days.Conclusion The modified method to establisha BALb/c mice of obstructive colon cancer model are operational simple,reduces intestinal injury,technically feasible,oncological safety,loss mortality,and higher stability.That has basically reflected pathological physiological evolution of clinical obstructive colon cancer,and are desired animal models.
出处
《大连医科大学学报》
CAS
2017年第6期584-588,共5页
Journal of Dalian Medical University
基金
辽宁省自然科学基金项目(201602037)
大连大学博士启动基金项目(20151QL019)
关键词
梗阻性结肠癌
小鼠模型
模型稳定性
obstructive colon cancer
BALb/c mice model
model stability
