摘要
目的比较正常小鼠和荷乳腺癌小鼠骨髓中髓系抑制性细胞(MDSCs)的含量,探讨其产生免疫抑制功能的可能机制。方法建立荷乳腺癌小鼠模型,利用BD FACSAriaⅢ流式分选仪分选正常小鼠组和荷乳腺癌小鼠组骨髓中的MDSCs(CD11b+Gr-1+),比较其含量;q-PCR法检测两组MDSCs中MCT1及LDHB的mRNA水平。结果荷乳腺癌小鼠骨髓中MDSCs的比例为(48.92±3.13)%高于正常小鼠(25.13±1.95)%,差异有统计学意义(P<0.001);荷乳腺癌小鼠MDSCs中MCT1的mRNA相对表达量(0.010±0.005)为正常小鼠(0.003±0.001)的3.33倍,差异有统计学意义(P<0.05);荷乳腺癌小鼠MDSCs中LDHB的mRNA相对表达量(0.008±0.002)为正常小鼠(0.004±0.001)的2.00倍,差异极显著(P<0.01)。结论 MDSCs在荷乳腺癌小鼠骨髓中的比例显著高于正常小鼠,其能量代谢关键分子MCT1、LDHB的表达也显著高于正常小鼠。MDSCs的能量代谢特点可能正是其发挥免疫抑制功能的重要基础。
Objective To test the MDSCs ratios in bone marrow from normal and breast cancer bearing mice,and compare the mRNA levels of the key energy metabolism molecules-MCT1/LDHB and explore the possible mechanism of immunosuppression in MDSCs.Methods MDSCs（CD11b＋Gr-1＋） in bone marrow was sorted by BD FACSAriaⅢ.MCT1/LDHB mRNA levels were detected with q-PCR.Results The MDSCs ratio（19.77±4.77） % in breast cancer bearing mice was much higher than that（0.84±0.18） % in normal group（P〈0.001）.Both MCT1 and LDHB mRNA of MDSCs in tumor bearing group were significantly higher than that in normal group（P〈0.05 and P〈0.01,respectively）.Conclusions The energy metabolism could play an role in the immunosuppression of MDSCs.
出处
《大连医科大学学报》
CAS
2017年第6期532-535,共4页
Journal of Dalian Medical University
基金
福建省卫计委青年课题(2016-1-60)
2016年福建医科大学启航基金(2016QH042)
