摘要
目的 评价右美托咪定对脓毒症小鼠体液免疫功能的影响.方法 SPF级健康雄性BALB∕c小鼠90只,1月龄,体重18～22 g,采用随机数字表法分为3组（n=30）：假手术组（S组）、脓毒症组（SEP组）和右美托咪定组（DEX组）.采用盲肠结扎穿孔法建立小鼠脓毒症模型,S组仅行剖腹探查术.DEX组于关腹后即刻腹腔注射右美托咪定30 μg∕kg,S组和SEP 组给予等容量生理盐水.于术后6、12和24 h（T1-3）时采集眼眶静脉血样,采用ELISA 法检测血清IgG、IgA、IgM 和补体C3、补体C4的浓度;随后取脾脏,采用MTT法检测脾脏B淋巴细胞增殖能力.结果 与S组比较, SEP组和DEX组T2,3时血清IgG、IgM 浓度和脾脏B淋巴细胞增殖能力降低,T1时脾脏B淋巴细胞增殖能力升高,T1-3时血清补体C3和补体C4浓度升高（P〈0.05）,血清IgA浓度差异无统计学意义（P〉0.05）;与SEP组比较,DEX组T2,3时血清IgG和IgM 浓度升高,T1,2时脾脏B淋巴细胞增殖能力升高,T1-3时血清补体C3和补体C4的浓度降低（P〈0.05）,血清IgA浓度差异无统计学意义（P〉0.05）.结论 右美托咪定可改善脓毒症小鼠体液免疫功能.
Objective To evaluate the effect of dexmedetomidine on humoral immune function in septic mice. Methods Ninety SPF healthy male BALB∕c mice, aged 1 month, weighing 18-22 g, were divided into 3 groups（n=30 each）using a random number table： sham operation group（group S）, sep-sis group（group SEP）and dexmedetomidine group（group DEX）. Sepsis was induced by cecal ligation and puncture in SEP and DEX groups, and group S only underwent exploratory laparotomy. Dexmedetomi-dine 30 μg∕kg was intraperitoneally injected immediately after peritoneum closure in group DEX, and the e-qual volume of normal saline was given instead in S and SEP groups. The orbital venous blood samples were collected at 6, 12 and 24 h after operation（T1-3）for determination of serum concentrations of IgG, IgA, IgM and complement C3 and C4 by enzyme-linked immunosorbent assay. Then the spleen was removed, and the proliferation of spleen B lymphocytes was detected by methyl thiazolyl tetrazolium assay. Results Compared with group S, the serum IgG and IgM concentrations and proliferation of spleen B lymphocytes were significantly decreased at T2,3, the proliferation of spleen B lymphocytes was enhanced at T1, the ser-um concentrations of complement C3 and C4 were increased at T1-3（P〈0.05）, and no significant change was found in serum IgA concentrations in SEP and DEX groups（P〉0.05）. Compared with group SEP, the serum concentrations of IgG and IgM were significantly increased at T2,3, the proliferation of spleen B lymphocytes was enhanced at T1,2, the serum concentrations of complement C3 and C4 were decreased at T1-3（P〈0.05）, and no significant change was observed in serum IgA concentration in group DEX（P〉0.05）. Conclusion Dexmedetomidine can improve humoral immune function in septic mice.
出处
《中华麻醉学杂志》
CSCD
北大核心
2017年第10期1271-1273,共3页
Chinese Journal of Anesthesiology
基金
甘肃省自然科学基金（17JR5RA262）
