摘要
目的研究肌球蛋白轻链激酶(MLCK)在哮喘小鼠气道中的表达及其对哮喘气道炎症和肺功能的影响。方法将20只雌性BALB/c小鼠随机分为对照组(n=10)及哮喘组(n=10),利用鸡卵白蛋白(OVA)建立小鼠哮喘模型。光学显微镜下计数血清中白细胞及嗜酸性粒细胞总数;ELISA方法检测肺泡灌洗液中炎性介质IL-4、IFN-γ水平;Western blotting法检测小鼠肺组织M LCK的表达;免疫实时荧光定量(q PCR)检测M LCK在基因水平的表达。结果肺功能检测示哮喘组小鼠最大呼气流量峰值(PEF)、第0.4秒用力呼气容积占用力肺活量比值(FEV0.4/FVC)均低于对照组(P均<0.001);哮喘组小鼠白细胞及嗜酸性粒细胞计数均高于对照组(P均<0.001);ELISA结果示哮喘组小鼠BALF中炎性介质IL-4浓度较对照组明显升高(P<0.001),而IFN-γ浓度较对照组降低(P<0.001);哮喘组小鼠肺组织MLCK表达量明显升高(P=0.006);与对照组相比,哮喘组MLCK在基因水平上的表达升高(P<0.001)。结论 MLCK可能参与哮喘气道炎症反应并影响肺功能。
Objective To study the effect of myosin light chain kinase（ MLCK） on airway inflammation and pulmonary function through the determination of its expression in asthmatic mice airway. Methods Twenty female BALB/c mice were randomly divided into normal control group（ n = 10） and asthma group（ n = 10）. Mice model of asthma was established by using chicken ovalbumin（ OVA）. The counts of leukocytes and eosinophilic granulocytes were observed with optical microscope. The expressions of the inflammatory mediators as IL-4 and IFN-γ in bronchoalveolar lavage fluid（ BALF） were detected by ELISA kit. The expression of MLCK in the lung tissue of asthmatic mice was detected by Western blotting. The expression of MLCK in the gene level was detected by q PCR. Results The lung function test showed that peak expiratory flow（ PEF）,the ratio of forced expiratory volume in 0. 4 second to forced vital capacity（ FEV0. 4/FVC） in asthma group was lower than those in normal control group（ P〈0. 001）. The counts of leukocytes and eosinophilic granulocytes in asthma group were higher than those in normal control group（ P〈0. 001）. The IL-4 level in the BALF in asthma group was higher than that in normal control group（ P〈0. 001）,while the IFN-γ levelwas lower than that in nornal control group（ P〈0. 001）. The expression of MLCK in the lung tissue of asthma group was significantly increased（ P = 0. 006）. The expression of MLCK in the gene level of asthma group was higher compared with the normal control group（ P〈0. 001）. Conclusion MLCK may be involved in the airway inflammation response and influence the lung function.
出处
《山东大学学报（医学版）》
CAS
北大核心
2017年第12期18-23,共6页
Journal of Shandong University:Health Sciences
