摘要
试验以费菜(Sedum aizoon L.)为材料,研究了不同浓度NaCl(0、8、12、16、20 g·kg^(-1))处理下接种GMH、GMA 28 d费菜幼苗的生长、叶绿素含量及叶绿素荧光参数变化。结果表明,当NaCl浓度在0~8 g·kg^(-1)时,随着盐处理浓度的增大,费菜叶绿素含量增加,初始荧光(Fo)增大,盐害指数增大,最大荧光(Fm)、光系统II(PSII)最大光化学效率(Fv/Fm)下降、实际光化学效率(ΦPSⅡ)减小,非光化学猝灭(NPQ)上升,叶片通过调节热耗散维持较高的光化光能量分配;光化学猝灭(q P)与PSII电子传递速率(ETR)降低,电子传递受到抑制。NaCl浓度超过8 g·kg-1时,光保护机制作用遭到破坏。接种AMF费菜根长、株高、根冠比、根系活力与对照CK相比均有显著增加。接种AMF植株的Fv/Fm得到明显提高,减轻了盐害对费菜电子传递速率和光能转化效率的影响,并配合通过增加热耗散来降低过剩激发能对PSII的伤害,同时维持尽量多的能量参与光化学反应,提高了费菜的光合能力,增强了费菜的盐耐受程度,但当NaCl浓度超过12 g·kg-1时,盐害同样也伤害费菜的PSII。同时,接种GMA提高费菜耐盐性的效果高于接种GMH。
Sedum aizoon L. seedlings were treated with NaCl( 0,8,12,16,20 g·kg^(-1)) in plastic pots with soil. We studied the effects of NaCl on growth,chlorophyll content and chlorophy II fluorescence parameters after 28 days. The results indicated that with the increase of NaCl concentration in the range of 0 g·kg^(-1) to 8 g·kg^(-1),Chlorophyll content,minimal fluorescence( Fo),and the salt injury index increased,while maximum fluorescence( Fm),photochemical maximum efficiency of PSII( Fv/Fm),actual photochemical efficiency( ΦPSⅡ),non-photochemical quenching coefficient( NPQ),and the ability of leaves to transform energy decreased compared with the control. In addition,photochemical quenching coefficient( q P) and electron transport rate( ETR) decreased.Treatment of 8 g·kg^(-1) NaCl caused damage of photo-protective mechanisms. After inoculation with different AMF,the root length,plant height,root-shoot ratio,root activity were significantly increased compared with the control.Fv/Fm was significantly increased under the stress by inoculated with AMF. Inoculation with AMF reduced the damage of electron transfer rate,light energy conversion efficiency,the damage to PSII resulted from the excess excitation energy by increasing thermal dissipation,and maintain as much as energy to participate in photochemical reactions. It improved the photosynthetic ability of Sedum aizoon L. and eventually enhanced plant resistance to salt stress. However,when the concentration exceeded 12 g ·kg-1,PSII also suffered from damages. Compared with GMH,GMA can improve the salt tolerance of Sedum aizoon L.
出处
《干旱地区农业研究》
CSCD
北大核心
2017年第6期134-139,153,共7页
Agricultural Research in the Arid Areas
基金
四川省教育厅项目(10ZB044)
关键词
NACL胁迫
费菜
AMF
叶绿素
荧光参数
盐害指数
NaCl stress
Sedum aizoon L.
arbuscular mycorrhizal fungus
chlorophyll
fluorescence parame ters
salt injury index
