摘要
目的研究聚维酮碘腹腔冲洗对结直肠癌大鼠血流动力学及酸碱平衡的影响。方法选取雄性SD大鼠30只,5周龄,给予大鼠腹腔单次注射氧化偶氮甲烷剂量为15 mg·kg-1,1周后予以大鼠饮用3个循环3%葡聚糖硫酸钠盐(3%葡聚糖硫酸钠饲喂7d+正常饮水14d为一个循环)制备结直肠癌模型。采用随机数字表法将结直肠癌大鼠分为3组:对照组(C组)、生理盐水组(N组)和聚维酮碘组(P组),每组10只。于第16周在造模鼠中每组随机选取两只大鼠肉眼及光镜下观察结直肠组织,以判断造模是否成功,病理提示造模成功后按组别给予相应处理。C组为假手术组,麻醉后行腹部正中切口手术;N组麻醉后行腹部正中切口,予以生理盐水7m L·kg-1冲洗腹腔;P组麻醉后行腹部正中切口,予以0.45%~0.55%聚维酮碘溶液7m L·kg-1冲洗腹腔;建立有创颈动脉测压后记录冲洗前(T0)、冲洗后5 min(T1)、冲洗10 min(T2)、冲洗后15 min(T3)、冲洗后20 min(T4)、冲洗后30 min(T5)的心率(HR)、平均动脉压(MAP),抽取大鼠颈动脉血检测冲洗前(T0)及冲洗后30 min(T5)时刻血气。结果与T0时比较,T1~T5时P组MAP、HR,T5时p H值、BE值、HCO3-浓度降低(P<0.05);T5时P组血LA浓度升高(P<0.05)。P组T1~T5时MAP、HR,T5时p H值、BE值、HCO3-浓度低于C组和N组(P<0.05);P组T5时血LA浓度高于C组和N组(P<0.05)。结论聚维酮碘溶液冲洗结直肠癌大鼠腹腔能降低p H值,引起乳酸升高,影响围术期血流动力学的稳定。
Objective To investigate the effect of peritoneal lavage with povidone-iodine on hemodynamic parameters and acid-base parameters in rats（SD）. Methods 30 male SD rats aged 5 weeks were established rat model of colorectal cancer.All rats were injected intraperitoneally with a single dose（15 mg·kg-1）of azoxymethan（AOM）followed by three cycles of DSS in drinking water for 1 week and normal drinking water for 2 weeks. They were randomly divided into three groups（n=10）： sham operation（group C）,0.9% saline solution（group N）,povidone-iodine（group P）. Sixteen weeks later,each group was selected two rats to check whether the models succeeded or not. Group C was sham operation group,after anesthesia,rats were made abdominal median incision without futher treatment.In group N,peritoneal lavage through median laparotomy was performed with 0.9% saline solution 7 m L·kg-1. In group P,peritoneal lavage through median laparotomy was performed with 0.45%-0.55%Povidone-iodine 7 m L·kg-1. MAP and HR before peritoneal lavage（T0）and 5 minutes（T1）,10 minutes（T2）,15 minutes（T3）,20 minutes（T4）and 30 minutes（T5）after peritoneal lavageof rats were monitored for carotid artery pressure. Results Compared to HR and MAP at T0,HR and MAP at T1~T5 and p H,BE,HCO3-at T5 in group P were significantly decreased（P〈0.05）,and LA at T5 was significantly increased（P〈0.05）. HR and MAP at T1~T5 and p H,BE,HCO3-at T5 in group P were significantly lower than those in group C and group N（P〈0.05）. LA at T5 was significantly higher than that in group C and group N（P〈0.05）. Conclusion Peritoneal lavage with povidone-iodine in experimental colorectal cancer rats can affect hemodynamics balance by decreasing p H and increasing LA during perioperative period.
作者
宋华丽
张冬梅
赵娜
郎淑慧
高玉华
SONG Huali;ZHANG Dongmei;ZHAO Na;LANG Shuhui;GAO Yuhua(Ningxia Medical University, Yinchuan 750004;Department of Anesthesiolgy,the General Hospital of Ningxia Medical University, Yinchuan 750004)
出处
《宁夏医科大学学报》
2017年第10期1124-1126,1132,共4页
Journal of Ningxia Medical University
基金
宁夏医科大学校级重点项目(XZ2015021)
关键词
聚维酮碘
腹腔冲洗
血流动力学
酸碱平衡
大鼠
povidone-iodine
peritoneal lavage
hemodynamics
acid-base balance
rat
