摘要
节球藻毒素-R(nodularin-R,NOD-R)是具有强烈肝毒性的蓝藻毒素。然而,现有的NOD-R检测技术均存在一定的局限性,亟待开发一种新的检测方法。本文利用指数富集的配基系统进化(systematic evolution of ligands by exponential enrichment,SELEX)技术结合高通量测序(HTSELEX),筛选出NOD-R特异的高亲和力适配体。生物膜干涉(biolayer interferometry,BLI)技术对适配体的鉴定结果表明,适配体H62与NOD-R之间的亲和力最高,达到了168 n M。而且,该适配体不与其他毒素结合,具有较高的特异性。以上结果表明,适配体H62有望作为NOD-R检测新方法——适配体生物传感器的分子识别元件。
Nodularin-R （NOD-R） , a eyanophycean toxin, is a different limitations of the detection methods for NOD-R. As a potent hepatotoxin. However, there are result, there is still an urgent need to develop a novel approach for the detection of NOD-R. Here, we combined High Throughput Sequencing （HTS） with Systematic Evolution of Ligands by Exponential Enrichment （SELEX） method （HT- SELEX）, and selected aptamer that bound with high affinity and specificity to NOD-R. Biolayer interferometry （BLI） assays showed that aptamer H62 was the best one targeting NOD-R. The binding affinity between aptamer H62 and NOD-R was as high as 168 nM. Furthermore, aptamer H62 bound with high specificity to NOD-R. These results indicate that aptamer H62 would be a new molecular recognition element and provide clues for the construction of aptasensor specializing in NOD-R detection.
出处
《中国生物化学与分子生物学报》
CAS
CSCD
北大核心
2018年第1期69-76,共8页
Chinese Journal of Biochemistry and Molecular Biology
基金
重大新药创制科技重大专项(No.2018ZX09J18112)资助~~
