摘要
为提高资源利用效率,降低微生物油脂发酵成本,解决微生物油脂发酵中废弃酵母细胞和发酵废液处理排放的问题,研究隐球酵母(Cryptococcus podzolicus)Zwy-2-3利用栎类淀粉发酵产油情况,并探讨发酵废液和废弃酵母细胞酶解液作为氮源的循环利用.结果显示,以葡萄糖60 g/L和总氮0.18 g/L的废弃酵母细胞酶解液发酵时,循环3次其油脂产量分别达到6.79 g/L、6.66 g/L、6.72 g/L,均高于对照组;而将发酵废液回收用作废弃酵母细胞酶解的缓冲液时,其生物量、油脂产量同对照组相当;将该方法应用于栎类淀粉水解液同步糖化发酵产油脂的实验,循环3次后其生物量、油脂产量分别为13.04 g/L、7.13 g/L,比对照组提高了9.85%、10.03%,且3次循环的油脂含量较为稳定.油脂组分分析结果显示,菌株Zwy-2-3利用栎类淀粉同步糖化和废弃细胞循环酶解液发酵生产的微生物油脂不饱和脂肪酸的含量达到93%以上,与植物油组成相似.综上,酶解废弃酵母细胞可有效应用于酵母产油发酵,可为非粮淀粉生产的微生物油脂应用于生物柴油生产奠定基础.
To improve the efficacy of resources, reduce the costs of microbial lipid production, and solve the problem of disposal of the spent cell mass and spent water, it is imperative to recycle resources, including spent cell mass, mineral nutrients, and spent water. In this study, lipid production by the oleaginous yeast Cryptococcus podzolicus Zwy-2-3 was used as a model system to demonstrate the recycling of an enzymatic solution of spent cell mass and spent water as a nitrogen resource for microbial lipid fermentation. It was found that when serial repitching experiments were performed with 60 g/L glucose and spent cell mass hydrolysates alone as a nitrogen resource, the fermentation produced 6.79 g/L, 6.66 g/L, and 6.72 g/L lipid yield in three successive cycles, which was higher than that in the control. In contrast, biomass and the lipid yield remained almost constant when spent water was also recycled. The method was applied to the simultaneous saccharification and fermentation of an acorn starch hydrolysate, and after three cycles, the biomass and lipid yield were 13.04 g/L and 7.13 g/L, the growth rates were 9.85% and 10.03%, respectively, and the lipid content of the three cycles was stable. Our results show efficient recycling of lipid production waste and should help to advance the microbial lipid technology
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2017年第6期979-983,共5页
Chinese Journal of Applied and Environmental Biology
基金
国家"十二五"科技支撑计划项目(2014BA D02B02)
国家微生物资源平台项目(NIMR-2016-8)
四川省科技厅项目(2014GZX0005
18ZDYF1802
2017TJPT0001)资助~~
关键词
栎类淀粉
隐球酵母Zwy-2-3
同步糖化发酵
氮源循环
acron starch
Cryptococcus podzolicus Zwy-2-3
simultaneous saccharification and fermentation
recycle nitrogen resource
