摘要
本实验旨在通过构建猪ANK1基因启动子序列的双荧光素酶报告基因系统,分析ANK1基因启动子活性,寻找ANK1基因启动子的核心区域,为下一步研究猪ANK1基因启动子序列SNP与活性之间的关系提供依据。利用Methprimer和Ali Baba2.1软件预测分析ANK1基因启动子区序列,预测其转录结合位点和Cp G岛。利用直接测序法,对广西巴马小型猪和长白猪共64个样品的ANK1基因启动子核心区域的单核苷酸多态性进行研究。双荧光素酶实验结果表明所预测的序列具有启动子活性,并寻找到启动子的核心区域;同时发现了16个SNP位点,它们分别是:-19、-133、-147、-187、-228、-246、-332、-360、-369、-468、-549、-550、-615、-696、-725和-798。
The aim of this study was to analyze the promoter activity of ANK1 gene and find the core of the promoter region of ANK1 gene by constructing the double luciferase reporter gene system of porcine ANK1 gene promoter, which could provide reference for the further research on the relationship between ANK1 gene promoter SNP and activity in pigs. The sequence, transcriptional binding site and Cp G island of transcription factors in the ANK1 gene promoter were predicted and analyzed by Methprimer and Ali Baba2.1 software. Sixty-four blood samples from Guangxi Bama mini-pig and landrace were collected to analyze the SNP of ANK1 gene promoter core region by direct sequencing. The result of dual luciferase reporting assay showed that the predicted sequence had obvious promoter activity, and the promoter core region was found. Meanwhile, 15 SNP sites in ANK1 gene promoter core region were detected, and they were-19,-133,-147,-187, 228,-246,-332, 360, 369,-468,-549,-550, 615,-696,-725, and-798.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2017年第12期5084-5089,共6页
Genomics and Applied Biology
基金
国家现代农业技术体系广西生猪创新团队项目(nycytxgxcxtd-03-15)资助
