摘要
目的通过观察山楂叶总黄酮(hawthorn leaf flavonoids,HLF)对大鼠心肌缺血再灌注损伤模型的影响,探讨其对缺血再灌注损伤心肌细胞的保护作用。方法选取Male Wistar大鼠40只,雌雄各半,随机分为正常对照组、模型组及HLF低剂量组、高剂量组,每组各10只;各组均正常饲养,HLF低剂量组、高剂量组分别灌胃给予山楂黄酮0.5 g/kg、1.0 g/kg每日1次,连续7 d;建立大鼠心肌缺血损伤模型,记录V3心电图,比较各组ST段上移、T波升高的幅度及出现心律失常的数量;心电图检查完成后,腹主动脉采血,取血清,测定心肌超氧化物歧化酶(SOD)、肌酸激酶(CK)水平。结果不同剂量的山楂黄酮均可改变实验性大鼠急性心肌缺血的心电图S-T段变化幅度,减少心律失常发生率,提高心肌SOD活力,抑制CK活性。结论 HLF对大鼠心肌缺血再灌注心肌损伤具有保护作用,其机制可能与抑制自由基生成,改善能量代谢等有关。
Objective To observe the effect of hawthorn leaf flavonoids（ HLF） on myocardial ischemia-reperfusion injury in rats,and to explore its protective effect on myocardial cells injured by ischemia-reperfusion. Methods Forty male Wistar rats were randomly divided into normal control group, model group, HLF low dose group and high dose group, with 10 rats in each group.The rats in each group were fed with normal diet, HLF low dose group, High-dose group were given intraperitoneal administration of hawthorn flavone 0.5 g/kg,1.0 g/kg once daily for 7 days; established myocardial ischemia injury model in rats, recorded V3 electrocardiogram, compared the ST segment up shift, T wave after the electrocardiogram examination was completed, the abdominal aorta blood was taken and the serum was taken. The levels of myocardial superoxide dismutase（ SOD） and creatine kinase（ CK） were measured.Results Different doses of hawthorn flavonoids can change the S-T amplitude of ECG in acute myocardial ischemia rats, reduce the incidence of arrhythmia, increase myocardial SOD activity and inhibit CK activity.Conclusion HLF has a protective effect on myocardial injury induced by myocardial ischemia-reperfusion in rats. The mechanism may be related to inhibition of free radical generation and improvement of energy metabolism.
出处
《中国地方病防治》
北大核心
2017年第8期854-855,共2页
Chinese Journal of Control of Endemic Diseases
关键词
山楂黄酮
缺血再灌注
心功能
心肌酶
Hawthorn flavonoids
Ischemia reperfusion
Cardiac function
Myocardial enzymes
