摘要
【目的】观察补肾活血化痰开窍方对耳鸣大鼠耳蜗螺旋神经节神经元(SGN)中N-甲基-D-天冬氨酸(NMDA)受体亚型1、2A、2B(NR1、NR2A、NR2B)表达的影响,并探讨其作用机制,以期为临床应用补肾活血化痰开窍方治疗耳鸣提供实验依据。【方法】将60只大鼠随机分为正常组,模型组,中药低、中、高剂量组,西药组6组,每组10只。采用大鼠腹腔注射水杨酸钠联合"饮水抑制"法建立大鼠耳鸣模型。造模成功后,中药低、中、高剂量组给予补肾活血化痰开窍方(剂量分别为5.5、11、22 g·kg-1·d-1)灌胃,西药组给予卡马西平(剂量为5 mg·kg-1·d-1)灌胃,模型组和正常组给予2 m L生理盐水灌胃,每天1次,连续治疗8周。采用免疫印迹法和实时定量聚合酶链反应(q RT-PCR)检测其耳蜗SGN中NR1、NR2A、NR2B在耳鸣大鼠的表达情况。【结果】与正常组比较,模型组NR1、NR2A、NR2B在模型组中表达升高,差异具有统计学意义(均P<0.05);与模型组比较,中药各剂量组耳蜗SGN中NR1、NR2A、NR2B的表达均明显降低(均P<0.05)。【结论】补肾活血化痰开窍方可有效缓解大鼠耳鸣,其机制可能与降低耳鸣大鼠耳蜗SGN中增高的NMDA受体亚型NR1、NR2A、NR2B表达有关。
Objective To observe the effect of Kidney-nourishing, Blood-activating, Phlegm-resolving and Resuscitation-inducing Decoction（KBPRD）on the expression of N-methyl-D-aspartate（NMDA）receptor subtype1,2 A,2 B（NR1,NR2 A,NR2 B）in the cochlear spiral ganglion neurons（SGN）of tinnitus rats and to explore its mechanism,thus to provide experimental evidence for the treatment of tinnitus with KBPRD. Methods Sixty rats were randomly divided into normal group,model group,western medicine group,and low-,middle-,and highdose Chinese medicine groups,10 rats in each group. Rats were given intraperitoneal injection of sodium salicylate combined with water deprivation to induce tinnitus model. After successful establishment of the model,the rats in low-,middle-,and high-dose Chinese medicine groups were given gastric administration of KBPRD in the dosage of 5.5,11,22 g·kg-1·d-1 respectively,the rats in western medicine group were given gastric administration of 5 mg·kg-1·d-1 of carbamazepine,and rats in the model group and normal group were given gastric administration of 2 m L of normal saline,once every day,treatment time covering 8 weeks. The expression levels of NR1,NR2 A,andNR2 B in the cochlear SGN was detected by immunoblotting and real-time quantitative reverse transcription-polymerase chain reaction（q RT-PCR）after 8 weeks of treatment. Results Compared with the normal group,the expression levels of NR1,NR2 A and NR2 B in the model group were increased,the difference being significant（P 0.05）.Compared with the model group,the expression of NR1,NR2 A and NR2 B in low-,middle-,and high-dose Chinese medicine groups were significantly decreased（P 0.05）.Conclusion KBPRD is effective on relieving tinnitus of rats,and its mechanism is correlated with lowering the increased expression of NR1,NR2 A and NR2 B in SGN of tinnitus rats.
出处
《广州中医药大学学报》
CAS
2018年第1期129-134,共6页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
湖北省卫生和计划生育委员会科研项目(编号:2013Z-B22)
武汉市卫生和计划生育委员会科研项目(编号:WZ15A06)
关键词
补肾活血化痰开窍方
耳蜗螺旋神经节神经元
NMDA
NR1
NR2A
NR2B
耳鸣
疾病模型
动物
大鼠
Kidney-nourishing, Blood-activating, cochlear spiral ganglion neurons
NMDA
ratsPhlegm-resolving and Resuscitation-inducing Decoction
NR1
NR2A
NR2B
tinnitus
disease models, animal
