摘要
目的:通过构建α-萘异硫氰酸脂(α-naphthylisothiocyanate,ANIT)诱导小鼠肝损伤模型,研究杠板归总黄酮提取物对小鼠肝脏的保护作用及其作用机制。方法:随机将60只昆明种小鼠分为6组,分别为空白对照组,病理模型组,阳性对照组(联苯双酯0.15 g/kg),杠板归总黄酮提取物高、中、低剂量组(1.2、0.6、0.3 g/kg)。除空白对照组外,采用腹腔注射ANIT的方法制造小鼠急性肝损伤模型,用杠板归总黄酮提取物连续灌胃9 d,禁食20 h后,眼球取血、取肝组织,测定肝组织中超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)、谷胱甘肽(glutathione,GSH)的含量及血清谷丙转氨酶(alanine aminotransferase,ALT)、天冬氨酸转氨酶(aspartate amino transferase,AST)、乳酸脱氢酶(lactate dehydrogenase,LDH)、总胆汁酸(total bile acid,TBA)、总胆红素(total bilirubin,TBIL)水平。结果:与模型组相比,杠板归总黄酮高剂量组小鼠血清ALT水平[(243.14±27.35)U/L]、AST水平[(345.02±24.01)U/L]、LDH水平[(505.97±68.56)U/L]、TBA水平[(112.02±17.16)nmol/m L]、TBIL水平[(15.08±4.09)nmol/m L]以及肝组织中GSH含量[(63.08±4.88)μmol/mg]和MDA含量[(3.55±0.32)nmol/mg]明显降低,差异具有统计学意义(P<0.05);中剂量组小鼠肝组织中血清ALT水平[(330.56±24.74)U/L]、AST水平[(438.60±32.51)U/L]、LDH水平[(597.90±66.90)U/L]、TBA水平[(144.62±16.37)nmol/m L]、TBIL水平[(27.36±6.51)nmol/m L]以及肝组织中GSH含量[(54.52±3.80)μmol/mg]和MDA含量[(5.65±0.35)nmol/mg]与模型组相比也明显降低,差异也具有统计学意义(P<0.05)。结论:杠板归总黄酮对ANIT诱导小鼠胆汁淤积性肝损伤有一定的疗效。
Objective：To investigate the protective effects and mechanisms of total flavonoids （TF） on α-naphthylisothiocyanate-in- duced （ANIT-indueed） cholestatic liver injury in mice. Methods：Sixty Kunming mice were evenly randomized into 6 groups, namely normal group, model group, biphenyl dimethylester group （0.15 g/kg）, high-dose, middle-dose, and low-dose TF groups （ 1.2,0.6,0.3 g/kg）. Except for the blank control group, the acute liver injury model was established by intraperitoneal injection of ANIT, and went gastric lavage with total flavonoids extracted from Polygonum knotweed L. for 9 consecutive days. After fasting for 20 hours,the liver tissue and the blood from eyeballs were taken out, and the content of SOD, MDA and GSH in liver tissue, as well as the serum level of ALT, AST, LDH, TBA, TBIL were measured. Results：Compared with the model group,Serum ALT levels in high dose group mice of total flavonoids extracted from Polygonum knotweed L[（243.14 ± 27.35） U/L], Levels of AST[（345.02 ± 24.01 ） U/L], Levels of LDH [（505.97 ± 68.56） U/L],TBA[（112.02 ± 17.16） nmol/mL],TBIL[（15.08 ±4.09） nmol/mL] and GSH contents in the liver[（63.08 ± 4.88） umol/mg] as well as MDA contents[（3.55 ± 0.32） nmo]/mg] were lower in high-dose TF group than in model group,The difference was statistically significant （P〈0.05）. Serum ALT levels in medium dose group mice[（330.56 ± 24.74） U/L], levels of AST [（438.60 ± 32.51） U/L],LDH[（597.90 ± 66.90） U/L],TBA[（144.62 ± 16.37） nmol/mL],TBIL[（27.36 ± 6.51） nmol/mL] and GSH contents in the liver[（54.52± 3.80） umol/mg] as well as MDA content[（5.65 ± 0.35） nmol/mg] were also lower in middle-dose TF group than in model group,the difference was also statistically significant（P〈0.05）. Conclusion：The total flavonoids extracted from P. knotweed exerts therapeutic effect on ANIT-induced liver in-jury with cholestasis in mice.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2018年第1期32-35,共4页
Journal of Chongqing Medical University
关键词
杠板归
总黄酮
保肝
胆汁淤积性
α-萘异硫氰酸脂
Polygonum knotweed L.
total flavonoids
hepatoprotective
cholestatic
α-naphthylisothiocyanate
