摘要
盐穗木(Halostachys caspica)是荒漠盐碱地广泛分布的盐生植物,具有极强的耐盐性。为揭示盐胁迫下盐穗木基因组层面的基因表达变化特性,通过对300和500 mmol·L^(-1)Na Cl胁迫3 h的盐穗木同化枝进行了转录组测序。有效序列组装共得到153 298条平均长度为643 bp的unigenes,进行GO和KEGG功能聚类,分别获得47个GO功能小类和118个KEGG通路。差异表达基因分析显示,短期低盐(300 mmol·L^(-1))响应基因有4 432个,高盐(500 mmol·L^(-1))响应基因有2 580个,两个胁迫的共差异基因有1 245个,主要富集在细胞过程、代谢过程和响应刺激等类别中。从短期盐胁迫下盐穗木转录组筛选出渗透调节和活性氧清除的相关基因,大多为上调基因。说明盐穗木能够通过促进渗透调节和增强活性氧清除提高短期的盐胁迫适应能力。
As a halophyte with strong salt tolerance, Halostachys caspica widely distributes in desert saline-alkali land. In order to reveal the genomic changes of gene expression under salt stress, transcriptome sequencing of H.caspica assimilating branches with treatments of 300 and 500 mmol·L^-1 NaCl for 3 h was performed. A total of 153 298 unigenes with an average length of 643 bp were obtained with clean reads assembled. The 47 subclasses and 118 KEGG pathways were enriched in the GO terms and KEGG pathways, respectively. Differentially expressed genes analysis showed that there were 4 432 and 2 580 unigenes in response to low salt （300 mmol·L^-1） and high salt （500 mmol·L^-1） in short-term stress, respectively. The 1 245 unigenes were the common differentially expressed genes in the two salt stresses. They were mainly enriched in cellular process, metabolic process and response to stimulus. The osmotic regulation and reactive oxygen species （ROS） scavenging genes were screened out, and most of them were up-regulated. Therefore, H.caspica could improve short-term salt stress adaptation by enhancing the osmotic adjustment and ROS scavenging.
出处
《植物研究》
CAS
CSCD
北大核心
2018年第1期91-99,共9页
Bulletin of Botanical Research
基金
新疆重点实验室专项资金资助项目(2014KL001)~~
关键词
盐穗木
盐胁迫
转录组
差异基因
渗透调节
活性氧
Halostachys caspica
salt stress
transcriptome
differentially expressed genes
osmotic adjustment
reactive oxygen species
