摘要
黑果枸杞Lycium ruthenicum为多年生野生灌木,由于其珍贵的营养价值和强非生物胁迫抗性受到广泛关注。为研究黑果枸杞钾离子(K+)/钠离子(Na+)选择性运输的分子机制,从转录组数据中筛选出拟外整流K+通道基因,提取黑果枸杞根系总RNA为模板,采用反转录聚合酶链式反应(RT-PCR)方法分离出该拟似SKOR基因。序列分析显示:该基因序列长2 448 bp,编码815个氨基酸。通过比对发现:该基因与拟南芥Arabidopsis thaliana等植物中已报道的SKOR基因编码的氨基酸序列同源性在60%以上,与几种茄科Solanaceae植物的SKOR同源性高达90%以上。进一步对该基因进行表达分析,结果显示:Lr SKOR被盐碱处理强烈诱导而被高盐处理微弱诱导,盐碱处理是高盐处理的6.81倍,是对照的23.79倍,说明Lr SKOR的表达受到盐以及盐碱胁迫调控,并且pH值对其表达水平有显著影响。
To determine the molecular mechanism of Lycium ruthenicum, a perennial shrub species that has attracted great interest in recent years due to its nutritional value and abiotic stress resistance, with its high salt tolerance capability, a potential outward-rectifying potassium channel gene was cloned based on a transcriptome sequencing database analysis. Analyses also included real time（RT）-polymerase chain reaction（PCR）, a homology comparison analysis, and a gene expression analysis. Results of the RT-PCR indicated that the length of this potential L. ruthenicum SKOR（Lr SKOR） gene was 2 448 bp encoding 815 amino acids. The homology comparison analysis showed that the SKOR gene shared more than a 60% amino acid sequence similarity with reported plants, such as Arabidopsis thaliana, and had over 90% similarity with other Solanaceae plants. Gene expression analysis with RT-PCR showed that the Lr SKOR gene was strongly induced by NaHCO_3 stress and weakly induced by Na Cl stress; its expression level with NaHCO_3 stress was 6.81 times higher than Na Cl stress and 23.79 times higher than the control. Thus, expression of Lr SKOR, regulated by both saline and saline-alkali stress, with pH imposed a strong influence on its expression. The results could help in Lr SKOR gene function prediction as well as lay a foundation for further research of high salinity stress response in L. ruthenicum.
出处
《浙江农林大学学报》
CAS
CSCD
北大核心
2018年第1期104-111,共8页
Journal of Zhejiang A&F University
基金
北京林业大学青年教师科学研究中长期项目(2016ZCQ05)
国家自然科学基金面上项目(31370597
31670610)
内蒙古自治区科技重大专项(2014)
