摘要
为了探讨半膜覆盖调控玉米生长的差异表达基因及分子机制,以‘浚单29’为材料,露地栽培作为对照,与半膜覆盖间隔种植,利用cDNA-AFLP技术,采用90对单引物筛选半膜覆盖下差异表达基因,并进行生物信息学分析。结果表明,利用90对单引物组合共扩增出2 298条差异片段,其中上调表达的有1 429条,占总条带数的45%;下调表达的有869条,占总条带数的23.7%;无差异表达的有877条,占总条带数的27.6%。选择重复扩增稳定的20条差异片段进行回收测序,经过Blast数据库对比和基因注释分析,再将所得的15条差异片段按功能分为细胞骨架相关基因(13.3%)、能量代谢相关基因(13.3%)、细胞挽救和防御相关基因(6.7%)、转录过程相关基因(40%)、胞内转运相关基因(6.7%)和未知功能基因(20%)6大类。分析其中一些重要的基因,如RPM1在逆境抗性中起作用,MYM锌指蛋白、BCAS2蛋白和EIF3B翻译起始因子都与调控转录翻译相关。通过c DNA-AFLP技术筛选了多个半膜覆盖下差异表达的基因,为揭示半膜覆盖影响玉米生长发育的分子机制提供了基础资料。
In order to investigate the molecular mechanism of the regulation of maize growth under partial plastic film-mulched cultivation, the differentially expressed genes were screened by 90 pairs of single primers using c DNA-AFLP technique, and bioinformatics analysis was carried out with Jundan 29 as the material under openfield-cultivation for the control and partial plastic film-mulched. The results showed that a total of 2 298 differential fragments were amplified by the 90 primer pairs, of which 1 429 were up-regulated（45% of the total number of bands）, 869 were down-regulated（ 23.7% of the total number of bands）. 877 genes were expressed without difference（27.6% of the total number of bands）. Twenty different differential fragments were selected and sequenced. Used Blast database comparison and gene annotation analysis, fitteen fragments could be divided into 6 groups according to the functions, namely cytoskeleton genes（13.3%）, energy metabolism gens（13.3%）, cell rescue and defense genes（ 6.7%）, transcription genes（ 40%）, intracellular transport related genes（ 6.7%） and unknown function（20%）. Some important genes were analyzed. For example, RPM1 protein plays a crucial role in adversity resistance, MYM zinc finger protein, BCAS2 protein and EIF3B are related to the regulation of transcription and translation. This study would provide basic data for revealing the molecular mechanism of partial plastic film-mulched affecting maize growth and development.
出处
《热带作物学报》
CSCD
北大核心
2017年第12期2310-2317,共8页
Chinese Journal of Tropical Crops
基金
内蒙古科技计划项目(No.20140169)
国家重点研发计划项目(No.22016YFD0300309)
关键词
玉米
栽培方式
半膜覆盖
CDNA-AFLP
差异表达
Maize
cultivation pattern
partial plastic film-mulched
cDNA-AFLP
differential gene expression
