摘要
本文探索了大蓟提取物联合抗生素对分枝杆菌属模式菌株耻垢分枝杆菌的协同抗菌作用,以期从药用植物中鉴定新型抗分枝杆菌活性天然产物或新型抗菌增效剂。通过水煮法、索氏提取法、超声波法获取大蓟粗提物,采用管碟法、96微孔板二倍稀释法对三种粗提物进行体外分枝杆菌药敏试验,观察大蓟粗提物的抗分枝杆菌活性及与抗生素的协同抗菌活性。结果显示大蓟粗提物单独作用时,饱和浓度的大蓟水提物未表现出抗菌活性;索氏提取物和超声波提取物表现出一定的抗菌活性(MIC值均为8 mg/mL)。大蓟超声波提取物与抗生素联合作用时,与氯霉素、卡那霉素、庆大霉素和红霉素均表现出协同作用,其中与氯霉素、红霉素表现为明显协同。本文首次发现大蓟中存在与抗生素有协同作用的抗菌增效剂,为从药用植物中鉴定天然抗菌产物及抗菌增效剂奠定了一定基础。
In order to identify new anti-myeobacterial active natural products or new antibacterial synergists from medici- nal plants,the anti-mycobacterial activity and synergetic antimicrobial effectof crude extract of Cirsium japonicum DC. was evaluated in this study. Boiled method, Soxhlet extraction method and ultrasonic methodwere used to extract whole plant of C. japonicum. Three kinds of extracts were subjected to antibacterial test in vitro by tube dish method and 96 mi- crotiter plate dilution method. The results showed that when C. japonicum extract was used alone, the saturated concentra- tion of C. japonicurn water extract had no antimicrobial activity;and the MIC values of the Soxblet extract and ultrasonic extract of C. japonicum were 8 mg/mL. The results showed that the antibacterial effect of ultrasonic extract of C. japoni- cum combined with the antibiotics was enhanced. Among them, ultrasonic extract of C. japonicum combined with chloram- phenicol and erythromycin had obvious synergistic antibacterial effect against Mycobacterium smegmatis with the FIC in- dex below 0.5. For the first time, this study has been found that there are antibacterial synergists in C. japonicum. It has laid a foundation for the identification of natural antibacterial products or antibacterial synergists from medicinal plants.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2017年第12期2087-2091,共5页
Natural Product Research and Development
基金
四川省教育厅项目(16ZB0299)
内江师范学院博士科研启动项目
关键词
大蓟
耻垢分枝杆菌
抗菌
抗菌增效剂
Cirsium japonicum DC.
Mycobacterium smegmatis
antibacterial
synergists
