摘要
目的了解吉林地区支原体肺炎患儿耐大环内酯类抗菌药物状况及探讨可能存在的耐药机制。方法收集来本院就诊的1 272例0岁~15岁急性呼吸道感染患儿的咽拭子标本。进行支原体快速培养与药敏试验;抽取耐红霉素的支原体培养液100例提取DNA,进行16S rRNA基因PCR扩增;抽取16S rRNA基因PCR扩增阳性标本30例进行23S rRNA基因PCR扩增、琼脂糖凝胶电泳、回收目的片段测序,测序结果与Gen Bank已登录的肺炎支原体标准株(M129)23S rRNA基因进行比对。观察其有无耐药基因位点的突变。结果吉林地区呼吸道感染的患儿肺炎支原体快速鉴定培养阳性率为35.30%;肺炎支原体耐药率最高的是红霉素(61.1%);耐药肺炎支原体19株的分子机制均为23S rRNA结构域V区2063位点A-G的突变。结论首次证明吉林地区耐药肺炎支原体的分子机制为23S rRNA结构域V区2063位点AG的突变。
Objective To investigate the status of macrolide-resistant antimicrobials in children with Mycoplasma pneumonia in Jilin area and to explore the possible mechanism of drug resistance. Methods A total of 1 272 throat swabs from children aged0 to 15 years with acute respiratory tract infection were collected from our hospital for mycoplasma rapid culture and drug susceptibility testing; 100 cases of erythromycin-resistant mycoplasma culture solution were collected for DNA extraction,16 S rRNA gene PCR was conducted for amplification; 30 cases of positive samples in 16 S rRNA gene PCR amplification were conducted for23 S rRNA gene PCR amplification,agarose gel electrophoresis,recovery of the target fragment was sequenced. The sequencing results were compared with the 23 S rRNA gene of Mycoplasma pneumoniae standard strain（ M129） registered in Gen Bank.Observe whether there were mutations in the drug-resistant gene loci. Results The positive rate of Mycoplasma pneumoniae in children with respiratory tract infection in Jilin was 35. 30%. Mycoplasma pneumoniae showed the highest resistance rate of erythromycin（ 61. 1%）. The molecular mechanism of 19 drug-resistant Mycoplasma pneumoniae was A-G at 2063 site in V region of23 S rRNA. Conclusion The molecular mechanism of Mycoplasma pneumoniae resistance in Jilin area was first demonstrated to be A-G mutation at 2063 site in V region of 23 S rRNA.
出处
《中国卫生检验杂志》
CAS
2017年第23期3357-3359,共3页
Chinese Journal of Health Laboratory Technology
