摘要
为了观察中药对猪链球菌的体外抑菌效果,试验将所选的42种常见中药按照常规法制备水提物,采用平板打孔法和试管二倍稀释法测定中药水提物对猪链球菌的体外抑菌作用。结果表明:在42种中药中有29种中药对猪链球菌有一定的抑菌作用,其中黄连水提物的抗菌活性最强,MIC为0.98 mg/m L;其次是黄柏、黄芩、丹参,MIC为3.91 mg/m L;有13种中药对猪链球菌无抑菌作用。说明不同中药对猪链球菌有不同的抑菌作用,其中黄连、黄柏、黄芩等抗菌活性较强的中药可进一步用于研究。
The aim of the present study was to observe the bacteriostatic effect of traditional Chinese medicine on Streptococcus suis in vitro. The aqueous extracts of 42 kinds of common traditional Chinese medicine were prepared according to conventional methods. The antibacterial activity of traditional Chinese medicine aqueous extract on Streptococcus suis in vitro was determined by punch method and tube dilution method. The results showed that the 29 kinds of Chinese herbal medicine had bacteriostatic effect on Streptococcus suis in 42 kinds of Chinese herbal medicine.The aqueous extract of Huanglian had the strongest antibacterial activity（ MIC = 0. 98 mg/m L）,followed by Hunagbai,Huangqin and Danshen（ MIC = 3. 91 mg/m L）. 13 kinds of Chinese herbal medicine had no bacteriostatic effect on Streptococcus suis. The results indicated that the different Chinese herbal medicines had different bacteriostatic effects on Streptococcus suis. Among them,Huanglian,Hunagbai,Huangqin and other traditional Chinese herbal medicines had strong antibacterial activity,which could be used for further study.
基金
农业部公益性行业(农业)科研专项"猪重大瘟病辨证论治关键技术研究与示范"(201403051-08)
关键词
中药
猪链球菌
体外抑菌
打孔法
最小抑菌浓度
抗菌活性
traditional Chinese medicine
Streptococcus suis
bacteriostasis in vitro
punch method
minimal inhibitory concentration
antibacterialactivity
