摘要
目的:探讨中药地锦草对Hela细胞增殖和凋亡作用的影响。方法:以人宫颈癌Hela细胞株为研究对象,采用MTT法检测地锦草对Hela细胞增殖抑制作用的影响,AO/EB染色法荧光倒置显微镜观察药物作用后凋亡小体的形成,Annexin V-FITC流式细胞术(FCM)检测细胞凋亡变化情况。结果:地锦草能够抑制Hela细胞的体外增殖,抑制程度随药物浓度50μg·m L^(-1)升高到200μg·m L^(-1),抑制率也由30.4%增大到61.1%,显示了良好的浓度依存关系(P<0.05);AO/EB染色法显示药物作用后凋亡小体形成,FCM法结果显示随着药物作用质量浓度由0增高到200μg·m L^(-1),细胞凋亡率也由0.76%升高到57.17%(P<0.05)。结论:地锦草能够抑制Hela细胞增殖。其作用可能是通过诱导Hela细胞凋亡和分化实现的。
Objective：To investigate the effects of Euphorbia humifusa on proliferation,apoptosis of Hela cervical cancer cells.Methods：The Hela cell line was used for the study.The inhibition of E.humifusa on the proliferation of Hela cells was assayed by MTT.Apoptotic cells was determined using the AO/EB staining,and cell apoptosis was detected by flow cytometry （FCM）.Results：E.humifusa could inhibit the growth of Hela cell,the inhibitory rate increased with the drug concentration by the 50 μg·mL-1 to 200 μg·mL-1,inhibition rate also increased from 30.4% to 61.1% in concentration dependent manners（P〈0.05）.FCM revealed that the percentage of cells that underwent apoptosis mounts from 0.76% to 57.17% as the treated concentration was elevated from 0 to 200 μg·mL^-1.Conclusion：E.humifusa can efficiently inhibit the proliferation of Hela cells,maybe by the way of leading to its apoptosis.
出处
《中国现代中药》
CAS
2017年第12期1713-1716,1727,共5页
Modern Chinese Medicine
基金
江西省卫计委科技计划(2015A079)
关键词
地锦草
HELA细胞
增殖
凋亡
Euphorbia humifusa
Hela cell
proliferation
apoptosis
