摘要
目的探讨CD133、趋化因子受体4(CXCR4)在胰腺癌中的表达及其临床意义,以及两者之间的临床关联性。方法采用免疫组织化学等方法检测53例手术切除胰腺癌标本中癌组织、癌旁非肿瘤组织、胰周淋巴组织及正常胰腺组织中CD133、CXCR4的阳性表达情况,分别比较两者阳性表达与临床特点的关系,并进一步分析CD133及CXCR4阳性表达有无临床关联性。结果CD133、CXCR4的表达阳性率分别为75.5%(40/53)、83.0%(44/53),CD133与CXCR4的共表达率为69.8%(37/53)。CD133、CXCR4的阳性表达与淋巴转移、病理分期、分化程度有关(P<0.05),CD133与CXCR4在蛋白表达水平上呈显著相关性(P<0.05)。CD133、CXCR4阳性表达组术后生存期明显短于阴性组(χ~2=4.675、5.527,P=0.031、0.019)。结论 CD133、CXCR4作为胰腺癌干细胞中的两个重要分子,与胰腺癌的发生发展及后期转移密切相关,并可作为判断临床预后的有效指标。
Objective To investigate the expression and significance of CD133 and CXCR4 in pancreatic cancer and their clinical relevance. Methods Immunohistochemistry was used to detect the expression of CD133 and CXCR4 in fifty-three resected pancreatic cancer tissues, pericarcinomatous normal tissues, peripancreatic lymph node tissues and the normal pancreas tissues. The correlations among the variety of expressions of CD133 and CXCR4 and pancreatic clinical pathologic characters were statistically analysed, respectively to investigate whether there was clinical relevance between the expression of CD133 and CXCR4. Results The positive expression rates of CD133 and CXCR4 in pancreatic cancer tissues were 75.5% （40/53） and 83.0% （44/53） respectively. Co-expression rate of CD133 and CXCR4 was 69.8%（37/53）. The positive expression rates of CD133 and CXCR4 were associated with lymph node metastases, TNM pathologic stage, tumor differentiation degree, respectively （P〈0.05）. And the positive expression of CD133 and CXCR4 in protein expression level had obviously clinical relevance （P〈0.05）. Consequently, the postoperation survival rates of CD133-positive and CXCR4-positive groups were significantly shorter than those of negative groups, respectively （P〈0.05）. Conclusion As two important moleculars in prancreatic cancer stem cells, both CD133 and CXCR4 play important roles in the tumorigenesis, development and late lymph node metastasis of pancreatic carcinoma, and also can be used as the effective biomarkers to judge the clinical prognosis.
出处
《中华普通外科学文献（电子版）》
2017年第6期375-379,共5页
Chinese Archives of General Surgery(Electronic Edition)
