摘要
目的 研究 B1 9病毒中国株独特区 VP1的基因变异 .方法 采用聚合酶链反应技术 (PCR)从两例中国再生障碍性贫血患儿血清中扩增 VP1独特区基因片段 ,将其与PUC1 9连接 ,酶切鉴定筛选阳性克隆后测序分析 .结果 从一个患儿的血清中扩增出约 480 bp目的片段 ,并成功构建PUC1 9- VP1质粒 ,测序结果显示 ,与国外已发表的 Wi株VP1独特区序列相比 ,有 2处核苷酸发生改变 ,并可致所编码的氨基酸变化 .结论 中国 B1 9病毒
AIM To obtain and sequence DNA clone of VP1 unique region of human parvovirus B19 from Chinese patients and to further study the genetic mutation of human parvovirus B19 of the Chinese strain. METHODS Two samples of VP1 unique region of HPVB19 were amplified from aplastic crisis serum of two Chinese children by PCR. The target fragment was linked with PUC19. Positive clones were chosen by identification of endonuclease cut and sequenced. RESULTS A target fragment of 480 bp was amplified by PCR from the serum samples of one patient. Compared with the published B19 VP1 unique sequences, two base pair and de duced amino acid were found changed. CONCLUSION There are two mutations in the nucleotide sequence encoding the unique region of VP1 of human parvovirus B19 of the Chinese strain.
出处
《第四军医大学学报》
北大核心
2002年第16期1464-1466,共3页
Journal of the Fourth Military Medical University
基金
国家自然科学基金资助项目 ( 39870 0 2 1)
关键词
细小病毒B19
分子克隆
序列分析
parvovirus B19, human
cloning molecular
sequence analysis
