摘要
目的:通过观察温肾通络止痛汤对去卵巢模型大鼠骨代谢的影响,探究其治疗绝经后骨质疏松症的机制。方法:选用3个月龄SD雌性大鼠72只,60只行双侧卵巢切除(OVX)后,随机分为模型组(OVX组),戊酸雌二醇组,温肾通络止痛汤高剂量组、中剂量组、低剂量组,其余12只为假手术组。通过骨密度和骨代谢生化指标测定,评价温肾通络止痛汤治疗绝经后骨质疏松症的效果。结果:温肾通络止痛汤可以防止去卵巢SD雌性大鼠胫骨骨密度降低,减少血清白细胞介素-6(IL-6)、核因子κB受体活化因子配基(RANKL)表达,提高血清骨保护素(OPG)水平,从而降低血清RANKL/OPG比值,达到抗骨质疏松的作用。结论:温肾通络止痛汤治疗绝经后骨质疏松症的作用机制主要为下调血清IL-6、降低RANKL/OPG比值,从而抑制破骨细胞活性,提高骨密度。
Objective：To observe the effect of Wenshen Tongluo Zhitong decoction（WTZD） on bone metabolism in ovariectomized rats and to explore its mechanism of treating postmenopausal osteoporosis（PMOP）. Methods： There were 72 3-month-old female SD rats,in which 60 rats were given bilateral ovariectomy,and were randomly divided into the ovariectomy group,estradiol valerate group,WTZD of high dose group,WTZD of medi- um dose group,and WTZD of low dose group,and the left 12 rats were the sham operation group. Bone mineral density（BMD） and biochemical indices for bone metabolism were measured to evaluate the efficacy of WTZD on PMOP. Results：In the PMOP female SD rats,WTZD can prevent the decrease of BMD, down-regulate IL-6 and receptor activator of nuclear factor kappa-B ligand（RANKL）,up-regulate osteoprotegerin（OPG） ,thus lowering RANKL/OPG ratio. WTZD had the effect of anti-PMOP. Conclusion：The main mechanism of WTZD PMOP is down-regulating IL-6,reducing RANKL/OPG ratio,which could inhibite osteoclast activity to improve BMD.
出处
《山东中医杂志》
2017年第12期1055-1058,1074,共5页
Shandong Journal of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(编号:81473692)
江苏省高校优势学科建设工程资助项目(2014.06)
南京中医药大学校级重点实验室专项基金资助项目(2015.09)
康缘中医药科技创新与奖励基金项目(编号:HZ1004KY)
关键词
温肾通络止痛汤
骨质疏松症
受体活化因子配基
骨保护素
Wenshen Tongluo Zhitong decoction
osteoporosis
receptor activator of nuclear factor kappa-B ligand
osteoprotegerin
