摘要
目的:探讨p21蛋白激活激酶4(PAK4)在乳腺癌细胞上皮间质转化中的作用。方法:在乳腺癌MCF7细胞中超表达PAK4,而在乳腺癌MDA-MB-231细胞中干扰PAK4表达,通过迁移和侵袭实验检测其对乳腺癌细胞迁移侵袭的影响,荧光定量法和免疫印迹分析检测PAK4、E-Cadherin、N-Cadherin、Vimentin mRNA和蛋白质表达量变化,免疫印迹法检测EMT相关转录激活因子Snail、Slug表达量变化。结果:MCF7细胞超表达PAK4后,细胞的迁移侵袭能力显著增加,并且上皮标志分子E-Cadherin蛋白表达下调,而间质标志分子N-Cadherin和Vimentin蛋白则表达上调,EMT相关转录激活因子Slug表达量增加;MDA-MB-231细胞中,抑制内源PAK4表达能显著降低细胞的迁移侵袭能力,上调E-Cadherin蛋白表达,下调NCadherin、Vimentin和Slug蛋白的表达。结论:PAK4通过上调Slug促进乳腺癌发生上皮间质转化,可作为抑制乳腺癌转移的分子靶点。
[Abstartc] Objetcive： To analyze the effect of p21 protein activated kinase 4（ PAK4） on epithelial-mesenchymal transition of breast cancer cells.Met hods： The mRNA and protein expression of PAK4,E-Cadherin,N-Cadherin and Vimentin were detected by qRTP-CR and Western blot in breast cancer cells transfected with pcDNA -PAK4 or siRNA PAK4;The biology behaviors of MCF-7 cells and MDA-MB-231 cells transfected with pcDNA-PAK4 or siRNA PAK4 were analysed by cell migration assay ,invasion assay.Results：Overexpressing PAK4 could significant increase in the migration and invasion compared with vector-infected cells, decrease the expression of epithelial marker E-cadherin and upregulate the expression of mesenchymal markers N-cadherin and Vimentin in detached MCF7 cells.Silenced PAK4 gene in detached MDA-MB-231 cells could suppress the migration and invasion ,decrease the levels of the mesenchymal markers and increased the levels of the epithelial markers at both mRNA and protein levels .Conclusion：PAK4 plays a key role in EMT of breast cancer cells ,and its promoting EMT effect associated with upregulating the expression of Slug .
作者
米旭光
李首庆
刘磊
芦小单
方艳秋
谭岩
MI Xu-Guang;LI Shou-Qing;LIU Lei;LU Xiao-Dan;FANG Yan-Qiu;TAN Yan
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2017年第12期1771-1773,1778,共4页
Chinese Journal of Immunology
基金
吉林省科技发展计划项目(20150520047JH)
吉林省科技厅重点实验室建设专项基金(20150622024JC
20160622008JC)
关键词
乳腺癌
p21蛋白激活激酶4
上皮间质转化
Breast cancer
p21 protein activated kinase 4
Epithelial-mesenchymal transition
