摘要
背景:前期研究通过相分离技术制备了细菌纳米纤维素/聚乙烯醇复合管,显著改善了单纯细菌纳米纤维素管的机械力学性质,但对复合管生物相容性的研究并不充分。目的:分析细菌纳米纤维素/聚乙烯醇复合管的生物相容性。方法:通过单硅胶管和双硅胶管两种生物反应器,在线培育获得两种不同结构细菌纳米纤维素管,分别记为S-BNC和D-BNC;采用相分离方法将两种细菌纳米纤维素管分别与聚乙烯醇复合,制备细菌纳米纤维素/聚乙烯醇复合管;通过溶血率、血浆复钙、血小板黏附实验评价S-BNC、D-BNC、S-BNC/聚乙烯醇复合管及D-BNC/聚乙烯醇复合管材料的血液相容性。将4种管材料分别与猪髋骨内皮细胞(或人血管平滑肌细胞)共培养,3,7 d后,采用活死细胞染色观察细胞在材料表面生长情况。结果与结论:①血液相容性:4组管的溶血率均小于0.5%。D-BNC管的血浆复钙时间显著快于S-BNC管(P<0.05);两复合管的血浆复钙过程较相应的单细菌纳米纤维素管均有减缓(P<0.05),两复合管血浆复钙时间无差异。血小板在两种纯细菌纳米纤维素管的内表面均发生了较大的形变,并且相互聚集,形成很多血小板栓子;两复合管的血小板黏附数量明显减少,且D-BNC/聚乙烯醇复合管血小板黏附量显著多于S-BNC/聚乙烯醇复合管;②细胞相容性:4组管材料均支持猪髋骨内皮细胞的生长,两复合管较对应的单细菌纳米纤维素管效果更好;4组管材料均支持人血管平滑肌细胞的生长,相对于单纯细菌纳米纤维素管,复合管可显著促进细胞的生长,其中以D-BNC/聚乙烯醇复合管的效果最好;③结果表明:细菌纳米纤维素/聚乙烯醇复合管具有良好的血液相容性与细胞相容性。
BACKGROUND： Based on our previous findings, the mechanical properties of bacterial nanocellulose （BNC） tubeswere improved by composite with polyvinyl alcohol （PVA） using phase separation method. However, the biocompatibilityof the composite tubes is rarely reported.OBJECTIVE： To evaluate the biocompatibility of BNC and BNC/PVA composite tubes.METHODS： Two types of BNC tubes （S-BNC and D-BNC） with different structures were online prepared in twobioreactors （single-silicone tube bioreactor and double-silicone tubes bioreactor respectively）. BNC/PVA compositeswere prepared based on both BNC tubes using a phase separation method. The hemocompatibility of these tubesincluding hemolytic ratio, plasma recalcification and platelet adhesion were compared. Living/dead staining method wasused to evaluate the cell growth after 3 and 7 days incubation since the endothelial and smooth muscle cells wereseeded on the lumen surfaces of the BNC and BNC/PVA composite tubes, respectively.RESULTS AND CONCLUSION： （1） Hemocompatibility： The hemolytic ratios of four kinds of tubes were all below 0.5%.The plasma recalcification time of D-BNC tube was significantly shorter than that of S-BNC tube （P 〈 0.05）. The plasmarecalcification processes of two composites were slowed as compared with corresponding BNC tubes （P 〈 0.05）. Nosignificant difference in plasma recalcification was detected between the two composites. The platelets adhered on theinner surface of two pure BNC tubes showed greater deformation and aggregated distribution, which led to the formationof platelet plugs. The platelet adhesion of BNC/PVA composites was dramatically reduced. The D-BNC/PVA compositetube showed more platelet adhesion than the S-BNC/PVA composite tube. （2） Cytocompatibility： Pig iliac endothelialcells could grow on the lumen surface of these four kinds of tubes. And the composites showed better growth ascompared with corresponding pure BNC tubes. Human vascular smooth muscle cells could also grow on the lum
出处
《中国组织工程研究》
CAS
北大核心
2017年第34期5474-5480,共7页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金面上项目(51373031)
上海市科委"科技创新行动计划"项目(15520720800)
纤维材料改性国家重点实验室资助课题(LK1617)~~
