摘要
目的探讨是否规律抗癫痫治疗对癫痫患者外周血T淋巴细胞水平变化的影响及意义。方法收集2016年9月至2017年8月黔江民族医院住院及门诊就诊的癫痫患者300例,根据是否服用抗癫痫药物分为未服药组和规律服药组,各150例,同时选取同期门诊健康体检者或志愿者150例为对照组。应用流式细胞仪检测3组患者外周血中CD3^+、CD4^+、CD8^+T淋巴细胞水平,计算CD4^+/CD8^+比值;采用免疫透射比浊法测定3组患者血浆免疫球蛋白IgG、IgA、IgM水平。结果与对照组比较,癫痫患者(未服药组+规律服药组)CD8^+T淋巴细胞比例升高,CD3^+、CD4^+T淋巴细胞比例及CD4^+/CD8^+比值降低(P<0.05,P<0.01);与未服药组比较,规律服药组CD3^+、CD4^+T淋巴细胞比例及CD4^+/CD8^+升高,CD8^+T淋巴细胞比例降低(P<0.05,P<0.01)。与对照组比较,癫痫患者(未服药组+规律服药组)血浆IgG、IgA等免疫球蛋白含量降低(P<0.05,P<0.01);与未服药组比较,规律服药组IgA含量升高(P<0.01)。结论癫痫患者体内普遍存在细胞和体液免疫功能紊乱,规律服用抗癫痫药物可改善免疫功能失衡现象。
Objective To investigate the effect and significance of regular anti-epileptic therapy on peripheral blood T lymphocyte levels in epileptic patients. Methods A total of 300 cases of epileptic patients who received treatment in Chongqing Qianjiang National Hospital from September 2016 to August 2017 were divided into non-medication group and regular medication group according to whether they took anti-epilepsy drugs （150 cases each） , and 150 cases of healthy examinees or volunteers at the same time were selected as control group. Flow cytometer was used to detect the level of CD3^＋ , CD4^＋ and CD8^＋ T lymphocytes in peripheral blood of 3 groups, and the ratio of CD^4＋ and CD8^＋ was calculated. Turbidimetric inhibition immuno assay was used to detect the plasma immunoglobulin IgG, IgA and IgM level in of 3 groups＇ patients. Results Compared to control group, the levels of CD8^＋ were higher in epileptic patients （ non-medication group and regular medication group）, and the level of CD3^＋ , CD4^＋ and the ratio of CD4^＋ and CD8^＋ were lower in epileptic patients （ P 〈 0.05, P 〈 0.01 ）. Compared to the non-medication group, the level of CD3^＋ , CD4^＋ and the ratio of CD4^＋ and CD8^＋ were higher in regular medication group, and the level of CD8^＋ in non-medication group was lower than that in regular medication group （ P 〈 0.05, P 〈0.01 ）. The level of plasma IgA and IgG in epileptic patients were lower than those in control group （ P 〈 0.05 ,P 〈 0.01 ）. The level of IgA in regular medication group was higher than that in non- medication group （ P 〈 0.01 ）. Conclusion Cellular and humoral immune dysfunction are generally exist in epileptic patients, and the regular use of anti-epileptic drugs can improve the imbalance of immune function.
出处
《中国临床研究》
CAS
2017年第12期1621-1623,共3页
Chinese Journal of Clinical Research
基金
重庆市黔江区科技计划项目(2016082)
关键词
癫痫
外周血T淋巴细胞
免疫球蛋白
规律服药
Epilepsy
Peripheral blood T lymphocyte
Immunoglobulin
Regular medication
