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结肠癌病灶内C/EBPα、FOXC2表达量的研究及其下游靶基因的初步分析

Study on C/EBPαand FOXC2 expression in colon cancer lesions and preliminary analysis of their downstream target genes
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摘要 目的:研究结肠癌病灶内C/EBPα、FOXC2的表达量并探究其下游靶基因。方法:选择2014年3月~2017年2月期间在陕西省渭南市中心医院手术切除的结肠癌标本,另取距肿瘤病灶边缘5cm以上的癌旁组织标本作为对照。抽提临床标本中的RNA并测定C/EBPα、FOXC2以及内质网应激分子、上皮间质转化分子的mRNA表达量。结果:结肠癌标本中C/EBPα、Bax、Bak、GRP78、PERK、ATF6的mRNA表达量显著低于癌旁标本,FOXC2、FAK、SphK1、N-cadherin、Vimentin的mRNA表达量显著高于癌旁标本;C/EBPα高表达的结肠癌标本中Bax、Bak、GRP78、PERK、ATF6的mRNA表达量显著高于C/EBPα低表达的结肠癌标本;FOXC2高表达的结肠癌标本中FAK、SphK1、N-cadherin、Vimentin的mRNA表达量显著高于FOXC2低表达的结肠癌标本。结论:结肠癌病灶内低表达的C/EBPα和高表达的FOXC2分别能够抑制内质网应激所介导的细胞凋亡、促进上皮间质转化所介导的细胞侵袭。 Objective:To study the C/EBPαand FOXC2 expression in colon cancer lesions and explore their downstream target genes.Methods:Colon cancer specimens surgically removed in Weinan Central Hospital between March 2014 and February 2017 were selected,and adjacent tissue specimens more than 5 cm from tumor lesion margin were selected as control.The RNA in the clinical specimens was extracted and the mRNA expression of C/EBPα,FOXC2 as well as endoplasmic reticulum stress molecules and epithelial-mesenchymal transition molecules were determined.Results:C/EBPα,Bax,Bak,GRP78,PERK and ATF6 mRNA expression levels in colon cancer specimens were significantly lower than those in adjacent samples while FOXC2,FAK,SphK1,N-cadherin and Vimentin mRNA expression levels were significantly higher than those in adjacent specimens;Bax,Bak,GRP78,PERK and ATF6 mRNA expression levels in colon cancer specimens with high C/EBPαexpression were significantly higher than those in colon cancer specimens with low C/EBPαexpression;FAK,SphK1,N-cadherin and Vimentin mRNA expression levels in colon cancer specimens with high FOXC2 expression were significantly higher than those in colon cancer specimens with low FOXC2 expression.Conclusion:Lowly expressed C/EBPαand highly expressed FOXC2 in colon cancer lesions can inhibit the apoptosis mediated by endoplasmic reticulum stress and promote the cell invasion mediated by epithelial-mesenchymal transition.
作者 蔡冰 宫惠琳
出处 《海南医学院学报》 CAS 2017年第21期2990-2993,共4页 Journal of Hainan Medical University
基金 陕西省科技攻关基金资助项目(2014-SF1-14)~~
关键词 结肠癌 CCAAT/增强子结合蛋白-α 叉头框蛋白C2 内质网应激 上皮间质转化 Colon cancer CCAAT/enhancer binding protein-α fork head box C2 endoplasmic reticulum stress epithelial-mesenchymal transition
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