摘要
为优化能稳定表达人酸性成纤维细胞生长因子(aFGF)的重组大肠杆菌的发酵条件,采用分批发酵方式,在菌体对数生长期通过添加IPTG诱导目的蛋白高效表达,确定aFGF工程菌较优发酵条件为:培养温度为37℃、培养基pH值为7.4、摇床转速为200 r/min。
Objective ： to optimize the condition for fermentation of an engineering E. coli strain which expressed human acidic fibroblast growth factor （aFGF） . Methods： the engineering E. coli strain with human aFGF gene was grown by batch fermentation, adding IPTG during the logarithmic growth phase supported a high expres-sion of target protein. Results：the optimal condition was confirmed,which was incubation at 37 Xl ,200 rpm and pH 7.4 of culture medium. Conclusion： the condition for fermentation of engineering E. coli strain with human aFGF gene was preliminarily optimized.
出处
《湖北理工学院学报》
2017年第5期51-54,共4页
Journal of Hubei Polytechnic University
基金
湖北理工学院校级科研重点项目(项目编号:17xjz07A)
关键词
酸性成纤维细胞生长因子
发酵
诱导
表达量
acidic fibroblast growth factor fermentation induction expression level
