摘要
研究比较了过滤性能良好的Scope大麦麦芽与存在过滤问题的单二麦芽的水溶性蛋白质组差异,发现参与阿拉伯木聚糖降解的阿拉伯木聚糖呋喃糖苷酶、阿魏酸酯酶存在较大差异,对水溶性阿拉伯木聚糖成分分析发现,单二麦芽中具有较高含量的阿魏酸,推测阿魏酸酯酶缺失是单二麦芽过滤性能缺陷的原因之一,通过添加纯化的阿魏酸酯酶,可有效提高麦汁的过滤速度,降低麦汁浊度和黏度。进一步研究发现,阿魏酸酯酶可有效去除阿拉伯木聚糖侧链上的阿魏酸,从而使得其氧化交联减弱,多聚阿拉伯木聚糖含量降低。
Filterability is one of the most important quality traits of malt.It is of great practical significance to resolve the problem of filterability defects of Chinese malt.The present study compared that proteomes of Scope and Dan＇er malts that with distinct filterability.It was found that arabinoxylan arabinofuranohydrolase and feruloyl esterase involved in the degradation of arabinoxylan were the most different enzymes.In addition,the components analysis of WEAX from Scope and Dan＇er malt revealed that WEAX from Dan＇er malt contained more ferulic acid.Therefore,we proposed that lack or short of feruloyl esterase in Dan＇er malt contributed majorly to its filterability defect.By adding purified recombinant feruloyl esterase in the mashing process using Dan＇er malt,the filtration rate of wort was significantly raised,accompanied by the decreased turbidity and viscosity of wort.The feruloyl esterase could remove the ferulic acid in the chain of arabinoxylan effectively and therefore inhibit the formation of ferulic acid dipolymer,which led to decreased content of poly-arabinoxylan.
作者
李晓敏
蔡国林
李兵
尹花
陆健
LI Xiao-min;CAI Guo-lin;LI Bing;YIN Hua;LU Jian(State Key Laboratory of Biological Fermentation Engineering of Beer, Qingdao 266100, China;National Engineering Laboratory of Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2017年第11期30-33,共4页
Food and Fermentation Industries
基金
国家863计划(2013AA102109)
啤酒生物发酵工程国家重点实验室开放基金资助课题(K2014001)
青岛创业创新领军人才计划项目(13-cx-15)
