摘要
目的初步探讨海南省不同性别百岁老人肠道菌群构成的差异。方法随机选取2016年4~9月海南省无消化道疾病的16例百岁老人(男女各8例),采集粪便样本,提取标本中原核生物全基因组后,采用16S rDNA高通量测序方法分析菌群构成。结果百岁老人样本操作分类单元(OTU)均值低于普通健康人群OTU均值;各组Shannon指数比较,女性组较于男性组差异有统计学意义(P=0.034);Lefse分析结果,女性组较于男性组厚壁菌门明显呈富集状态。结论百岁老人肠道菌群构成在整体上相对于普通健康人群简单,埃希氏菌属的相对丰度占绝对优势,百岁老人人群中女性较男性肠道菌群有着更好的多样性和均匀度。
Objective To investigate the differences of intestinal microflora in different sexes in Hainan Province. Methods According to the inclusion criteria, 16 cases of centenarians（8 cases of male and female）who had no gastrointestinal diseases in Hainan Province were randomly selected from April 2016 to September 2016. Stool samples were collected, and the whole prokaryotic genome was extracted. The strain composition was analyzed by 16 S rDNA high-throughput sequencing. Results The OTU mean of the centenarians was lower than that of the normal healthy group. There were significant differences in the Shannon index between the male group and female group（P=0.034）. The results of LEf Se analysis showed that Firmicutes in the female group was significantly enriched compared to that in the male group. Conclusion The composition of intestinal microflora in the centenarians is relatively simple compared with that of the healthy population. The relative abundance of Escherichia was dominant in the centenarians, and the females in the centenarians exhibited a more diverse population of intestinal flora than the male centenarians.
出处
《中华保健医学杂志》
2017年第6期467-470,共4页
Chinese Journal of Health Care and Medicine
基金
全军保健课题专项(14BJZ11)
海南省医药卫生科研项目(14A210195&14A210190)
海南省三亚市医疗卫生科技创新项目(2014YW42)
