摘要
目的:探讨铁皮枫斗颗粒治疗亚硝基胍(MNNG)诱发慢性萎缩性胃炎(CAG)模型大鼠的萎缩逆转作用。方法:Wistar大鼠自由饮用浓度为167μg/m L的MNNG水溶液,建立慢性萎缩性胃炎大鼠模型。确定成模后,将造模成功的大鼠分为模型组、铁皮枫斗颗粒组、叶酸片组,另设没有经过处理的大鼠为正常组。模型组灌胃生理盐水,铁皮枫斗颗粒组灌胃铁皮枫斗颗粒为1.2 g/(kg·d),叶酸片组灌胃叶酸片为5.0 mg/(kg·d)。观察大鼠体质量、胃组织病理组织学情况。结果:与正常组比较,模型组大鼠在治疗前和治疗第8周时体质量显著降低,炎症等级较高,腺体数量明显减少,腺体厚度明显降低,主细胞明显减少,胃组织出现不典型增生和肠化生的数量较多,差异有统计学意义(P<0.05,P<0.01)。与模型组比较,治疗第8周时,铁皮枫斗颗粒组和叶酸片组大鼠的体质量显著升高,炎症等级降低,腺体数量明显增多,腺体厚度明显升高,胃组织不典型增生和肠化生现象明显好转,不典型增生和肠化生的数量明显减少,差异均有统计学意义(P<0.05,P<0.01)。与叶酸片组比较,铁皮枫斗颗粒组大鼠的体质量较高,差异有统计学意义(P<0.05)。结论:铁皮枫斗颗粒与叶酸片均对CAG模型大鼠具有良好的治疗作用,可以减轻大鼠体质量丢失,减轻胃黏膜炎症等级、腺体萎缩、不典型增生。
Objective： To study the atrophy reversal effect of Tiepi Fengdou granules in treating model rats with chronic atrophic gastritis （CAG） induced by N-methyI-N＇-nitro-N-nitrosoguanidine（MNNG）. Methods： Wistar rats were fed with 167 μg/mL of MNNG water solution to establish model rats of CAG. After modeling, divided the model rats into the model group, Tiepi Fengdou granule group and folic acid tablets group, while untreated rats were in normal group. By gavage, the model group was given saline solution, Tiepi Fengdou granule group was given 1.2 g/（kg .d） of Tiepi Fengdou granules and folic acid tablets group was given 5.0 mg/（kg .d） of folic acid tablets. Observed body mass and situation of gastric tissue histopathology of the rats. Results= Compared with those in the normal group, in the model group, weight of rats was decreased remarkably, inflammation grade was higher, the number of inherent glands and gland thickness were remarkably reduced, the chief cells were remarkably decreased, and the number of atypical hyperplasia and intestinal metaplasia in gastric tissue was increased before treatment and after 8-week-medication, differences being significant（P〈 0.05, P〈 0.01）. Compared with the model group, in Tiepi Fengdou granule group and folic acid tablets group, the levels of body mass were significantly increased, levels of inflammation were decreased, the number of inherent glands and gland thickness evdently was increased, the situdation of atypical hyperplasia and intestinal metaplasia in gastric tissue was better and their number was evidently decreased after 8-week-medication, differences being significant（P 〈 0.05, P 〈 0.01）. Compared with that in folic acid tablets group, body mass in Tiepi Fengdou granule group was higher, the difference being significant（P〈0.05）. Conclusion. Tiepi Fengdou granules and folic acid tablets have a greatly curative effect on CAG rats models, which can reduce the loss of body mass and the level of gastric mucosa inflammation, gla
出处
《新中医》
CAS
2017年第12期7-10,共4页
New Chinese Medicine
基金
浙江省重大科技专项资助项目(2009C13032)
浙江省自然科学基金项目(LY15H280011)
国家科技重大专项重大新药创制课题资助项目(2016ZX09101069)
关键词
慢性萎缩性胃炎
铁皮枫斗颗粒
叶酸片
胃组织不典型增生
肠化生
动物实验
大鼠
Chronic atrophic gastritis., Tiepi Fengdou granules., Folic acid tablets., Gastric metaplasia in gastric tissue
Intestinal metaplasia
Animal experiment
Rats
