摘要
目的探讨碳青霉烯类药物单用或联合常用的抗被膜药物阿奇霉素对鲍曼不动杆菌生物被膜的作用。方法微量肉汤法检测亚胺培南、美罗培南对鲍曼不动杆菌浮游菌、被膜菌的敏感性,观察不同浓度的药物单药或联合阿奇霉素(AZI)时对鲍曼不动杆菌被膜的影响,用结晶紫实验观察被膜量的变化,用被膜下细菌计数的方法检测被膜细菌活力的改变。结果 4×MIC浓度的亚胺培南、美罗培南可以降低部分鲍曼不动杆菌的被膜量,剩余被膜量在17%~70%之间,被膜下细菌活力也有降低。与AZI联用时,鲍曼不动杆菌剩余被膜量较单用亚胺培南、美罗培南相比反而有所升高。结论碳青霉烯类药物可以有效降低细菌的被膜量及被膜下细菌,与阿奇霉素联用并不能增强其对被膜的活性。
Objective To investigate the effect ofcarbapenem alone or in combination with the commonly used anti-biofilm drug azithromycin on the biofilm ofAcinetobacter baumannii. Methods The susceptibility of imipenem and meropenem to Acinetobacter baumannii planktonic and biofilm bacteria were observed by the broth microdilution method. The effects of imipenem （IMP）, meropenem （MERO） monotherapy or the combination with azithromycin （AZI） on the biofilm of Acinetobacter baumannii were confirmed by the use of the crystal violet experiment to observe the changes in the amount of biofilm, and counting the bacteria under the biofilm to detect bacterial changes. Results IMP and MERO of 4~MIC could decrease the biomass of Acinetobacter baumannii biofilms, and the amount of residual biofilm was between 17% and 70%. The number of bacteria under the biofilm was also decreased. When combined with AZI, the amount of residual biofilm biomass was increased compared to carbapenem alone. Conclusion Carbapenems can effectively reduce the biomass ofAcinetobacter baumannii biofilms and the bacterial viability under the biofilm. Moreover, azithromycin in combination with carbapenem can not enhance the activity of imipenem and meropenem against biofilms ofAcinetobacter baumannii.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2017年第12期1040-1045,共6页
Chinese Journal of Antibiotics
基金
国家自然科学基金(No.81570008)
广东省自然科学基金(No.2014A030313052)
中山大学科技成果转化类项目(No.2017-9)
