摘要
目的探讨正常足月儿母亲胎盘菌群的结构和组成.方法采集足月经阴道正常分娩的胎盘组织3份,应用组织DNA提取试剂盒提取胎盘组织菌群的DNA,用16Sr RNA基因的高通量技术对样本进行测序,并对测序结果进行分析.结果在细菌门水平,5个菌门被检测出,即变形杆菌,未分类细菌门,放线菌门,厚壁菌门和拟杆菌门.大部分序列属于变形杆菌门,在胎盘组织中相对含量超过90%.在细菌属水平,有24个细菌属被检测到,主要属于变形杆菌门、放线菌门及厚壁菌门.胎盘中以变形杆菌门中的盐单胞菌属、Pelagibacterium和未分类的叶杆菌属为主.胎盘中也能检测到放线菌、大肠杆菌、志贺氏菌、单胞菌、多形杆状菌、链球菌、克雷伯氏菌、双歧杆菌、梭菌和葡萄球菌等.结论正常足月儿胎盘组织中有较丰富的细菌定植.
Objective To study the composition of the placenta microbiota of normal term delivery.Method Three placenta specimens were collected from full-term infant birth by normal spontaneous delivery.The flora DNA was extracted by Tissue Kit for 16SrRNA gene high-throughput nucleotide sequencing,then the sequencing results were analyzed.Results The placenta microbiota of normal spontaneous delivery at the bacterial phylum level showed five bacterial phylum were detected,including Proteobacteria,Unclassified Bacteria,Actinobacteria,Firmicutes and Bacteroidetes.Proteobacteria was the main parts of bacteria and more than 90 percent in the placenta microbiota.At the genus level,24 bacterial genus were detected including Proteobacteria,Actinobacteria and Firmicutes.Halomona,Pelagibacterium and Unclassified Phyllobacteriacea of Proteobacteria genus was the main bacterial genus in the placenta microbiota.Actinotalea,Escherichia/Shigella,Sphingomonas,Bacteroides,Streptococcus,Klebsiella,Bifidobacterium,Clostridium and Staphylococcus.Conclusion There are abundant bacterial flora colonized in the normal term placenta.
出处
《昆明医科大学学报》
CAS
2017年第11期52-57,共6页
Journal of Kunming Medical University
基金
国家自然科学基金资助项目(81360068)
"十二五"云南省特色学科群建设项目(201009)
关键词
胎盘
菌群
高通量测序
Placenta
Microbiota
High-throughput nucleotide sequencing
