摘要
目的探讨凋亡抑制因子(Apoptosis repressor with caspase recruitment domain,ARC)对双磷酸盐作用下的人成骨细胞的影响。方法建立ARC过表达的人成骨细胞,然后,用不同浓度的唑磷酸处理,MTT法用来检测细胞的增殖,流式细胞技术用来检测细胞凋亡情况,ALP染色及半定量等用来检测过表达ARC的成骨细胞的成骨分化。RT-PCR检测成骨相关基因BSP,Runx2,OCN及ALP的表达。结果 ARC能抑制唑磷酸引起的成骨细胞的凋亡,促进成骨细胞的成骨相关基因表达,促进成骨细胞的成骨分化。结论 ARC在调节唑磷酸处理的人成骨细胞中发挥了重要的作用。
Objective To investigate the effects of apoptosis repressor with caspase recruitment domain （ARC） on apoptosis and osteogenesis of zoledronic acid treated human osteoblast cells. Methods We established ARC-overexpressed human osteoblasts and exposed them to Zoledronate with different concentrations （0 μmol/ L, 1 μ mol/L,5 μmol/L） in vitro. Cell viability were detected using MTT assay, and cell apoptosis was measured by flow cytometry. ALP staining, quantitative analysis and ectopic osteogenesis in nude mice were used to evaluate the osteogenesis differentiation of ARC-overexpressed osteoblasts. RT-PCR was conducted to evaluate the expression of BSP, Runx2,OCN and ALP. Results ARC can reverse the inhibitory effect of Zoldronate on osteoblasts. ARC can also promote osteogenesis differentiation of osteoblasts and inhibit their apoptosis. Conclusion ARC play a critical role in the regulation of human osteoblasts under Zoledronate treatment.
出处
《口腔材料器械杂志》
2017年第4期184-190,共7页
Chinese Journal of Dental Materials and Devices
基金
上海市科委科技创新计划(15411950300)
