摘要
螺旋藻多糖和银杏叶提取物都具有抗衰老、抗氧化、抗自由基等多种生理功能,已发现二者合用具有独特药理活性,但关于它们对小鼠缺氧应激能力影响的研究很少。本研究旨在对不同比例复合螺旋藻多糖与银杏提取物的耐缺氧应激作用机理进行探讨。以小鼠为研究对象,将螺旋藻多糖(PSP)与银杏叶提取物(GBE)按不同比例复合,以100 mg/(kg·d)剂量分别灌服小鼠,连续给药14 d,在小鼠常压密闭缺氧模型中观察缺氧存活时间,检测心、肝、脑组织MDA含量,肝、脾SOD活力等氧化与抗氧化指标。结果发现,不同比例PSP与GBE均可延长小鼠在常压密闭缺氧条件下的存活时间,提高缺氧小鼠肝、脾SOD活力,降低缺氧小鼠心、肝、脑组织MDA含量。PSP与GBE以2∶1比例复合时抗缺氧应激的作用最好。上述结果表明,PSP与GBE复合有一定的抗缺氧应激作用,且以2∶1的比例协同增效作用最好,这可能是通过提高器官组织的抗氧化能力实现的。
Spirulina polysaccharide and Ginkgo biloba extract have many physiological functions, such as anti-aging,anti-oxidation and anti-free radicals. However,the combined effects of the two on hypoxia stress in mice are still unknown. This study was conducted to investigate the mechanism of action of different effects of Spirulinaplatensfs polysaccharides（PSP）and Ginkgo biloba extract （GBE）on hypoxia stress. The mice were used and PSP was combined with GBE leaves at different ratios in this experiment. Mice were fed with a intragastric administration in a dose of 100 mg/（kg·d）for 14 d,and were treated with a normobaric hypoxia model to observe the survival time of hypoxia. The content of MDA in the heart,liver and brain tissues,and SOD activity in the liver and spleen were detected. In result, the survival time of mice with different ratios of PSP to GBE could be prolonged,the SOD activity of the liver and spleen in hypoxia mice was increased,and the content of MDA in heart,liver and brain was decreased. The best ability to resist hypoxia stress was 2：1 compound PSP to GBE. These results indicated that PSP and GBE had anti-hypoxia stress,and the synergistic effect of 2 ： 1 was best,which may be achieved by improving the antioxidant ability of organs and tissues .
出处
《中国兽医科学》
CAS
CSCD
北大核心
2017年第12期1595-1601,共7页
Chinese Veterinary Science
基金
国家自然科学基金项目(31502138
31572590)
山东省自然科学基金项目(BS2015NY001)
山东省高校计划项目(J15LF03)
关键词
螺旋藻多糖(PSP)
银杏叶提取物(GBE)
耐缺氧
应激
polysaccharides from Spirulina platensis（PSP）
ginkgo biloba extracts（GBE）
hypoxia tolerance
stress
