摘要
建立了超高效合相色谱法测定尼龙6食品接触材料中己内酰胺迁移量的方法。样品经水、4%乙酸(V/V)、50%乙醇(V/V)、正己烷等4种食品模拟物浸泡后,50%乙醇、正己烷浸泡液直接进样,水、4%乙酸经乙醇稀释后进样,采用超临界CO2-甲醇(含2%水)为流动相进行梯度洗脱,经C18色谱柱(3.0 mm×100 mm,1.8μm)分离,通过PDA检测器对目标化合物在210 nm波长下进行分析,外标法定量。在优化条件下,己内酰胺在水、4%乙酸、50%乙醇、正己烷4种食品模拟物中,质量浓度范围分别在5.0~50.0 mg/L,5.0~50.0 mg/L,2.0~50.0 mg/L,5.0~50.0 mg/L线性良好,方法检出限分别为2.0,2.0,1.0,2.0 mg/L。进行添加回收实验,己内酰胺回收率为91.4%~98.4%,相对标准偏差为1.9%~3.2%。方法可用于食品接触材料中己内酰胺迁移量的快速测定。
The method for the determination of migration of caprolactam in nylon6 food contact material was developed. After the immersion of samples in four food simulants, 50% ethanol （volume fraction） and hexane were directly injected into the UPC2, while water and 4% acetic acid （volume fraction） were diluted by ethanol prior to injection. Caprolactam was separated on the column of C18 column （3.0 mm × 100 mm, 1.8 μm） by gradient elution with supercritieal carbon dioxide and methanol （containing 2% water） system, and analyzed under 210 nm with PDA. Under the optimized condition, the results of external standard method showed good linearities in the range of 5.0 50.0 mg/L for water , 5.0 - 50.0 mg/L for 4% acetic acid , 2.0 - 50.0 mg/L for 50% ethanol and 5.0 -50.0 mg/L for hexane, and the limits of detection were 2. 0, 2. 0, 1. 0 and 2. 0 mg/L, respectively. The recoveries were 91.4% - 98.4% and relative standard deviations （RSDs） （ n = 6） were 1.9% -3.2%. This method is simple, environment-friendly and accurate for rapid analysis of caprolaetam in food contact material.
出处
《分析试验室》
CAS
CSCD
北大核心
2017年第12期1435-1438,共4页
Chinese Journal of Analysis Laboratory
基金
国家质检总局科研项目(2016IK225)资助
