摘要
L-异亮氨酸在紫外光区无吸收峰,需将其进行衍生后才能测定.通过对比两种柱前衍生方法,建立高效液相色谱法柱前衍生检测L-异亮氨酸质量分数的方法.OPA自动衍生法检测耗时15 min,R=1,加标回收率99%~100.1%,RSD<1.0%;PITC手动衍生法检测耗时30 min,加标回收率96%~98%,RSD<1%.OPA自动衍生法在检测的时效性和准确性方面均具有明显优势,可用来高效地检测L-异亮氨酸的质量分数,在生物发酵、饲料添加剂、食品添加剂和制药等领域具有较高的应用价值.
L-Isoleucine has no absorption in the ultraviolet(UV) region and needs to be derivatized before determination by UV detector.The aim of this work is to compare the two pre-column derivatization methods: OPA automatic derivatization and PITC manual derivatization,and establish an efficient method for the determination of L-isoleucine content by high performance liquid chromatography.The total detection time with OPA automatic derivatization method took 15 minutes with R-value of 1,and the recoveries were from 99% to 100.1% with RSD < 1%.The PITC manual derivatization took 30 min,with the recovery rate of 96% to 98%(RSD < 1%).OPA automatic derivatization exhibited obvious advantages in the detection timeliness and accuracy,which can be used to efficiently detect L-isoleucine content.It has high application value in microbial fermentation,feed additives,food additives and pharmaceuticals.
出处
《发酵科技通讯》
CAS
2017年第4期220-223,共4页
Bulletin of Fermentation Science and Technology
基金
河北省氨基酸工程技术研究中心创新平台建设资助项目(131000021)
