摘要
目的探讨非小细胞肺癌（NSCLC）组织中长链非编码RNAHCG11的表达及临床意义。方法采用实时定量PCR方法检测146例新鲜NSCLC组织标本及相对应的癌旁正常组织标本中HCG11的表达，分析其与NSCLC的临床病理特征关系以及诊断价值和预后关系。结果与癌旁正常组织相比，在NSCLC组织中HCG11的表达明显降低（t=6．23，P〈0．01）；HCG11低表达和高表达组患者在肿瘤大小、病理类型方面差异均有统计学意义（χ2值分别为26．5123、9．5226，P值均〈0．05）；受试者工作特征曲线下面积为0．7887（95％CI：0．7105～0．8032，P〈0．001）；敏感度和特异度分别是59．6％和88．4％，表明具有潜在的诊断价值；生存分析表明HCG11低表达组总生存时间显著低于高表达组（χ2=5．517，P〈0．05）。结论HCG11在NSCLC中是低表达的，可以作为NSCLC的新型生物标记物和诊断靶标。
Objective To probe the expression and clinical significance of HCG11 in non-small cell lung cancer （NSCLC）. Methods Quantitative real time polymerase chain reaction was used to analyze HCGll expression in 146 cases of NSCLC tissues and adjacent normal tissues. The relationship of HCGll with clinicopathological characteristics, prognosis and diagnostic value in NSCLC was further explored. Results HCGll expression was remarkably decreased in the NSCLC tissues compared with the adjacent normal tissues （ t = 6.23, P % 0.01 ）. There were significant differences in clinicopathological characteristics such as tumor size, histological types between low HCG11 expression group and high HCGll expression group （χ2 = 26. 512 3, 9. 522 6, all P 〈 0.05）. The area under receiver operating characteristic curve was 0. 788 7 （95 % CI ： 0. 710 5-0. 803 2, P 〈0. 001）. The sensitivity and specificity was 59.6% and 88.4%, indicating that HCG11 had the potential diagnostic value. Survival analysis showed that overall survival was lower in the low HCGll expression group than that in the high HCGll expression group （ χ2= 5. 517, P 〈0.05）. Conclusions The expression of HCGll is down-regulated in NSCLC and it may be a new biomarker for diagnosis and prognostic significance in patients with NSCLC.
出处
《国际呼吸杂志》
2017年第22期1685-1688,共4页
International Journal of Respiration
