摘要
目的观察低能激光照射（LLLI）对大鼠心肌梗死区及其边缘组织中微小RNA（miRNA，miR）-214表达的影响并探讨其意义。方法将110只SD大鼠随机分为假手术组、对照组和治疗组。对照组和治疗组通过结扎左冠状动脉前降支制作心肌梗死模型，假手术组同部位只穿线不结扎，3周后治疗组使用LLLI处理梗死区域，对照组和假手术组照射同一区域但治疗仪不接通电源，各组分别于照射后1、24、48、72 h、7 d时取梗死区域组织，使用实时定量反转录聚合酶链反应（RT-qPCR）法检测miR-214的表达。结果治疗组梗死区miR-214的表达从LLLI处理后1 h开始上升（36.15±1.21），48 h达到峰值（58.95±1.34），7 d时表达量接近对照组（44.12±2.17），在1、24、48、72 h、7 d的表达均高于对照组（36.15±1.21比14.55±1.51, P＝0.000）、（52.14±1.32比21.22±0.46, P＝0.000）、（58.95±1.34比36.08±2.24, P＝0.000）、（53.85±1.34比38.01±1.95, P＝0.000）、（44.12±2.17比39.41±3.13, P＝0.000）。结论LLLI处理梗死心肌能明显上调梗死区域组织中miR-214的表达，延缓心肌纤维化，减轻病理性重塑，有较好的心肌保护作用。
ObjectiveTo observe the effect and significance of low-level laser irradiation （LLLI） on microRNA （miRNA, miR）-214 expression in myocardial infarct area and the edge of the organization.MethodsThe 110 SD rats were randomly divided into sham group, control group and treatment group. The control and treatment groups by ligation of the left anterior descending coronary artery produced myocardial infarction model, sham group wearing the same site without ligation, three weeks after the treatment group LLLI treatment infarct region, the control group and the sham group irradiating the same area but treatment device does not power on, each group 1, 24, 48, 72 h, take infarct tissue 7 d, using real-time quantitative reverse transcriptase-polymerase chain reaction （RT-qPCR） was used to detect expression of miR-214, respectively, after irradiation.ResultsThe expression treatment group, the infarct zone miR-214 from the rear L starts to rise for 1 h （36.15±1.21）, 48 h peak （58.95±1.34）, the expression of close control when 7 d （44.12±2.17）, in 1, 24, 48, 72 h, 7 d expression were higher （36.15±1.21 vs. 14.55±1.51, P=0.000）, （52.14±1.32 vs. 21.22±0.46, P=0.000）, （58.95±1.34 vs. 36.08±2.24, P=0.000）, （53.85±1.34 vs. 38.01±1.95, P=0.000）, （44.12±2.17 vs. 39.41±3.13, P=0.000）.ConclusionLLLI treatment can significantly increase the expression of myocardial infarction, infarct tissue miR-214, delaying myocardial fibrosis, reduce pathological remodeling, better myocardial protection.
出处
《中华实验外科杂志》
CSCD
北大核心
2017年第12期2177-2179,共3页
Chinese Journal of Experimental Surgery
基金
河南省科技厅科级攻关项目（162102310538）
郑州市科技创新人才培育计划领军人才资助项目（10URC175）
