摘要
目的探讨不同核酸检测方法在非淋球菌性尿道炎病原体检测中的临床价值。方法对173例疑似非淋球菌性尿道炎感染患者尿液以及尿道分泌物样本运用RNA恒温扩增技术(RNA-SAT)和实时荧光定量PCR技术(FQ-PCR)分别进行解脲支原体(Uu)和沙眼衣原体(Ct)检测,并对结果统计分析。结果 2种方法检测,尿道分泌物样本的Uu阳性率均高于尿液样本,差异有统计学意义(χ~2=4.011,P<0.05),而Ct阳性率经χ~2检验比较差异无统计学意义(χ~2=0.25,P>0.05)。Uu-尿液检测的敏感度(100.0%)高于Uu-尿道拭子(88.0%),差异有统计学意义(χ~2=9.049,P<0.01)。Ct-尿液和Ct-尿道拭子,二者敏感度差异无统计学意义(χ~2=0.642,P>0.05)。结论 RNA-SAT法既具备了FQ-PCR的高敏感度和特异度,又能很好地判断预后效果,同时可采用尿液作为待检样本,具有取样方便、耗时短、污染小及结果准确等优点,可用于临床实验室检测与疗效监测。
Objective To compare the application of the different nucleic acid test methods in the detection of non-gonococcal urethritis. Methods A total of 173 patients with suspected non-gonococcal urethritis were treated with RNA simultaneous amplification and testing（ RNA-SAT） and real-time fluorescence quantitative PCR（ FQ-PCR）,and the test results were conducted for statistical analysis. Results With the 2 detection methods,the positive rate of Uu of urethral discharge samples was higher than urine samples,and the difference was statistically significant（ χ~2= 4. 011,P〈 0. 05）. However,there is no statistical significance on the test differences of Ct positive rate by χ~2 test（ χ~2= 0. 25,P 〉0. 05）. The sensitivity of Uu-urine test（ 100%）was higher than Uu-urethral swab（ 88. 0%）,and the differences were statistically significant（ χ~2= 9. 049,P〈 0. 01）. The sensitivity difference between Ct-urine and Ct-Urethral swab was not statistically significant（ χ~2= 0. 642,P〉 0. 05）. Conclusion RNA-SAT method not only has the high sensitivity and specificity of FQ-PCR,but also can judge the prognosis effect well,at the same time,urine can be used as the sample to be tested. It has the advantages of convenient sampling,short time,little pollution and accurate result and other advantages,so it can be used for the clinical laboratory testing and efficacy monitoring.
出处
《中国卫生检验杂志》
CAS
2017年第22期3242-3243,3252,共3页
Chinese Journal of Health Laboratory Technology
关键词
非淋球性尿道炎
支原体
核酸扩增技术
感染
different nucleic acid test methods
non-gonococcal urethritis
non-gonococcal urethritis
RNA simultaneous amplification and testing（ RNA-SAT）
