摘要
目的分析前列腺素F2α受体(PTGFR)基因在发育性髋关节发育不良(DDH)患儿髋关节组织中的表达变化及其与DDH发病的相关性。方法 8对年龄相近、性别相同的DDH患儿(DDH组)和对照儿童(对照组)入组进行比较。采用实时定量PCR和蛋白质印迹法(Western blot)检测两组患儿PTGFR mRNA和蛋白表达水平。结果 DDH组患儿髋关节关节囊和圆韧带中PTGFR mRNA表达水平较对照组明显降低(t=3.472、2.887,P<0.05)。DDH组患儿髋关节关节囊和圆韧带中PTGFR蛋白表达水平较对照组明显降低(t=5.488、3.942,P<0.05)。结论 PTGFR可能在DDH发病中发挥重要作用,PTGFR可能是DDH的致病基因之一。
Objective To analyze the expression change of PTGFR gene in hip joint tissues of development dysplasia of the hip（DDH）and its correlation with DDH pathogenesis.Methods Eight age-and gender-matched children with DDH（DDH group）and control children（control group）were enrolled for conducting the compaison.The real-time quantitative PCR method and Western-blot method were adopted to detect the PTGFR mRNA and protein expression levels.Results PTGFR mRNA expression level in the hip articular capsule and ligamentum teres of the DDH group were significantly decreased compared with those of the control group（t=3.472,2.887,P〈0.05,）.The PTGFR protein expression level in the hip articular capsule and ligamentum teres of the DDH group were significantly decreased compared with those of the control group（t=5.488,3.942,P〈0.05）.Conclusion PTGFR could play an important role in DDH pathogenesis and may be one of DDH pathogenic genes.
出处
《重庆医学》
CAS
北大核心
2017年第33期4613-4615,共3页
Chongqing medicine
基金
国家自然科学基金项目(81401763)
上海卫生计生委科研项目(20144Y0176)
上海市科学技术委员会科研项目(12DZ2295006)
上海交通大学医学院“985”计划基金(YBKL2013008)
