摘要
目的研究东北刺人参Oplopanax elatus的亲水性成分及其抗结直肠癌活性。方法利用大孔树脂,正、反相硅胶柱及制备HPLC色谱方法分离纯化,根据理化性质和波谱数据鉴定化合物结构;并进行HCT-116和HT-29细胞增殖活性测定。结果从东北刺人参干燥根提取物正丁醇部位分离并鉴定了11个酚苷化合物,其中6个苯丙素苷成分:3,5-二甲氧基肉桂酸-4-O-β-D-葡萄糖苷(1)、3-羟基二氢肉桂醇-4-O-β-D-葡萄糖苷(2)、3-甲氧基肉桂醇-4-O-β-D-葡萄糖苷(3)、3-甲氧基二氢肉桂醇-4-O-β-D-葡萄糖苷(4)、3,5-二甲氧基二氢肉桂醇-4-O-β-D-葡萄糖苷(5)、3,5-二甲氧基肉桂醇-4-O-β-D-葡萄糖苷(6);5个木脂素苷成分:3,3′-二甲氧基-4,9,9′-三羟基-4′,7-环氧-5′,8-木脂素-4,9-O-β-D-二葡萄糖苷(7)、(+)-5,5′-二甲氧基落叶松脂素-4′-O-β-D-葡萄糖苷(8)、(+)-异落叶松脂素-9′-O-β-D-葡萄糖苷(9)、(+)-异落叶松脂素-4-O-β-D-葡萄糖苷(10)、(+)-5,5′-二甲氧基落叶松脂素-9′-O-β-D-葡萄糖苷(11)。所有酚苷对HCT-116和HT-29细胞增殖均无明显抑制作用(IC50>100μmol/L)。结论化合物4、6、9、11均为首次从该植物中分离得到,化合物1、2和10首次从该属植物中分离得到。
Objective To investigate the hydrophilic constituents from the anti-colorectal cancer extract of Oplopanax elatus. Methods The compounds were isolated and purified using macroporous resin, silica gel, ODS gel and pre-HPLC, and their chemical structures were identified by spectral data and physicochemical properties. The extracts and compounds from O. elatus were screened for anti-proliferation on HCT-116 and HT-29 cancer cell lines. Results Eleven phenolic compounds had been purified and identified from the n-butanol fraction including six phenylpropanoid glycosides:(E)-sinapic acid-4-O-β-D-glucopyranoside(1), 3-hydroxyphenethyl alcohol-4-O-β-D-glucopyranoside(2), 3-methoxycinnamyl alcohol-4-O-β-D-glucopyranoside(3), homovanillyl alcohol-4-O-β-D-glucopyranoside(4), dihydrosyringin(5), and syringin(6); And five lignan glycosides: 3,3′-dimethoxy-4,9,9′-trihydroxy-4′,7-epoxy-5′,8-lignan-4,9-bis-O-β-D-glucopyranoside(7),(+)-5,5′-dimethoxylariciresinol 4′-O-β-D-glucopyranoside(8),(+)-isolariciresinol-9′-O-β-D-glucopyranoside(9),(+)-isolariciresinol-4-O-β-D-glucopyranoside(10), and(+)-5,5′-dimethoxylariciresinol-9′-O-β-D-glucopyranoside(11). All the phenolic glycosides showed no significant effects on the proliferation of HCT-116 and HT-29 cancer cell lines with IC50 〉100 μmol/L. Conclusion Compounds 4, 6, 9, and 11 are isolated and purified from this herb for the first time, while compounds 1, 2, and 10 are firstly obtained from the genus Oplopanax.
出处
《中草药》
CAS
CSCD
北大核心
2017年第22期4620-4625,共6页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金青年基金资助项目(31400306)
中国博士后科学基金资助项目(2015M570692)